6-NED azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	615.78
Solvent	DMSO

Spectral properties

Absorbance (nm)	544
Extinction coefficient (cm ^-1 M ^-1)	74000¹
Excitation (nm)	545
Emission (nm)	567

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

6-NED maleimide

6-NED alkyne

Overview SDS Protocol

Molecular weight

615.78

Absorbance (nm)

544

Extinction coefficient (cm ^-1 M ^-1)

74000¹

Excitation (nm)

545

Emission (nm)

567

5'-Labeled primer pairs include a fluorescently labeled oligo with a choice of dye on the 5' end, along with an unlabeled oligo, in each pair. These products can be used in a variety of molecular biological applications. 6-NED is one of the colors used for making the well-known multicolor fluorescent oligo set of 6-FAM, 6-TET, 6-VIC, 6-HEX, 6-NED and 6-PET for use in fragment analysis, gene expression studies, genotyping, and PCR applications. 6 -NED azide is quite stable fluorescent probe that can be readily used for labeling alkyne-modified oligos (including DBCO-modified oligos).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-NED azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	162.396 µL	811.978 µL	1.624 mL	8.12 mL	16.24 mL
5 mM	32.479 µL	162.396 µL	324.791 µL	1.624 mL	3.248 mL
10 mM	16.24 µL	81.198 µL	162.396 µL	811.978 µL	1.624 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	544
Extinction coefficient (cm ^-1 M ^-1)	74000¹
Excitation (nm)	545
Emission (nm)	567

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM Azide	493	517	83000	0.178
6-HEX azide	533	559	73000	0.15
6-TET azide	521	542	73000	0.13
6-JOE azide	520	545	75000¹	-
6-TAMRA azide	552	578	90000	0.178
6-ROX azide	578	604	82000	0.168
6-NED, SE [6-NED NHS ester]	545	567	74000¹	-
6-OG488 Azide	498	526	76000	0.12
6-VIC Azide	526	543	-	-

Images

Figure 1. Chemical structure for 6-NED azide.

References

View all 22 references: Citation Explorer

Assessment of genetic diversity and bottleneck in Purnathadi buffaloes using short tandem repeat markers.
Authors: Ali, S Sajid and Kuralkar, S V and Das, Ramendra and Raina, Varinder and Kataria, R S and Vohra, Vikas
Journal: Animal biotechnology (2020): 1-12

Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82

HLA Class III: A susceptibility region to systemic lupus erythematosus in Tunisian population.
Authors: Hachicha, Hend and Mahfoudh, Nadia and Fourati, Hajer and Elloumi, Nesrine and Marzouk, Sameh and Feki, Sawsan and Fakhfakh, Raouia and Frikha, Faten and Ayadi, Abir and Maatoug, Amira and Gaddour, Lilia and Hakim, Feiza and Bahloul, Zouheir and Makni, Hafedh and Masmoudi, Hatem and Kammoun, Arwa
Journal: PloS one (2018): e0198549

Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22

Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57

Efficient multiplex simple sequence repeat genotyping of the oomycete plant pathogen Phytophthora infestans.
Authors: Li, Ying and Cooke, David E L and Jacobsen, Evert and van der Lee, Theo
Journal: Journal of microbiological methods (2013): 316-22

[Genetic polymorphisms of 15 X-STR loci in Shandong Han population].
Authors: Ma, Teng and Xu, Jing and Yang, Ya-Jun and Sun, Kuan and Xue, Fu-Zhong and Jin, Li and Li, Shi-Lin
Journal: Fa yi xue za zhi (2013): 202-5, 208

A new molecular approach to help conclude drowning as a cause of death: simultaneous detection of eight bacterioplankton species using real-time PCR assays with TaqMan probes.
Authors: Uchiyama, Taketo and Kakizaki, Eiji and Kozawa, Shuji and Nishida, Sho and Imamura, Nahoko and Yukawa, Nobuhiro
Journal: Forensic science international (2012): 11-26

A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables.
Authors: Elizaquível, Patricia and Aznar, Rosa
Journal: Food microbiology (2008): 705-13

Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36