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6-NED azide

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Physical properties
Molecular weight615.78
Spectral properties
Absorbance (nm)544
Extinction coefficient (cm -1 M -1)740001
Excitation (nm)545
Emission (nm)567
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
6-NED maleimide
6-NED alkyne


Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
5'-Labeled primer pairs include a fluorescently labeled oligo with a choice of dye on the 5' end, along with an unlabeled oligo, in each pair. These products can be used in a variety of molecular biological applications. 6-NED is one of the colors used for making the well-known multicolor fluorescent oligo set of 6-FAM, 6-TET, 6-VIC, 6-HEX, 6-NED and 6-PET for use in fragment analysis, gene expression studies, genotyping, and PCR applications. 6 -NED azide is quite stable fluorescent probe that can be readily used for labeling alkyne-modified oligos (including DBCO-modified oligos).


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-NED azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM162.396 µL811.978 µL1.624 mL8.12 mL16.24 mL
5 mM32.479 µL162.396 µL324.791 µL1.624 mL3.248 mL
10 mM16.24 µL81.198 µL162.396 µL811.978 µL1.624 mL

Molarity calculator

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Spectral properties

Absorbance (nm)544
Extinction coefficient (cm -1 M -1)740001
Excitation (nm)545
Emission (nm)567

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-HEX azide533559730000.15
6-TET azide521542730000.13
6-JOE azide520545750001-
6-TAMRA azide552578900000.178
6-ROX azide578604820000.168
6-NED, SE [6-NED NHS ester]545567740001-
6-OG488 Azide498526760000.12
6-VIC Azide526543--



View all 22 references: Citation Explorer
Assessment of genetic diversity and bottleneck in Purnathadi buffaloes using short tandem repeat markers.
Authors: Ali, S Sajid and Kuralkar, S V and Das, Ramendra and Raina, Varinder and Kataria, R S and Vohra, Vikas
Journal: Animal biotechnology (2020): 1-12
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
HLA Class III: A susceptibility region to systemic lupus erythematosus in Tunisian population.
Authors: Hachicha, Hend and Mahfoudh, Nadia and Fourati, Hajer and Elloumi, Nesrine and Marzouk, Sameh and Feki, Sawsan and Fakhfakh, Raouia and Frikha, Faten and Ayadi, Abir and Maatoug, Amira and Gaddour, Lilia and Hakim, Feiza and Bahloul, Zouheir and Makni, Hafedh and Masmoudi, Hatem and Kammoun, Arwa
Journal: PloS one (2018): e0198549
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
Efficient multiplex simple sequence repeat genotyping of the oomycete plant pathogen Phytophthora infestans.
Authors: Li, Ying and Cooke, David E L and Jacobsen, Evert and van der Lee, Theo
Journal: Journal of microbiological methods (2013): 316-22
[Genetic polymorphisms of 15 X-STR loci in Shandong Han population].
Authors: Ma, Teng and Xu, Jing and Yang, Ya-Jun and Sun, Kuan and Xue, Fu-Zhong and Jin, Li and Li, Shi-Lin
Journal: Fa yi xue za zhi (2013): 202-5, 208
A new molecular approach to help conclude drowning as a cause of death: simultaneous detection of eight bacterioplankton species using real-time PCR assays with TaqMan probes.
Authors: Uchiyama, Taketo and Kakizaki, Eiji and Kozawa, Shuji and Nishida, Sho and Imamura, Nahoko and Yukawa, Nobuhiro
Journal: Forensic science international (2012): 11-26
Clinical validation of a new triplex real-time polymerase chain reaction assay for the detection and discrimination of Herpes simplex virus types 1 and 2.
Authors: Reil, Heide and Bartlime, Ariane and Drerup, Jana and Grewing, Thomas and Korn, Klaus
Journal: The Journal of molecular diagnostics : JMD (2008): 361-7
A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables.
Authors: Elizaquível, Patricia and Aznar, Rosa
Journal: Food microbiology (2008): 705-13