6-NED azide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 615.78 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 544 |
Extinction coefficient (cm -1 M -1) | 740001 |
Excitation (nm) | 545 |
Emission (nm) | 567 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
6-NED maleimide |
6-NED alkyne |
Overview | ![]() ![]() |
See also: Cell Structures and Organelles, Nucleus, Digital PCR, DNA and RNA Quantitation, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
Molecular weight 615.78 | Absorbance (nm) 544 | Extinction coefficient (cm -1 M -1) 740001 | Excitation (nm) 545 | Emission (nm) 567 |
5'-Labeled primer pairs include a fluorescently labeled oligo with a choice of dye on the 5' end, along with an unlabeled oligo, in each pair. These products can be used in a variety of molecular biological applications. 6-NED is one of the colors used for making the well-known multicolor fluorescent oligo set of 6-FAM, 6-TET, 6-VIC, 6-HEX, 6-NED and 6-PET for use in fragment analysis, gene expression studies, genotyping, and PCR applications. 6 -NED azide is quite stable fluorescent probe that can be readily used for labeling alkyne-modified oligos (including DBCO-modified oligos).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-NED azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 162.396 µL | 811.978 µL | 1.624 mL | 8.12 mL | 16.24 mL |
5 mM | 32.479 µL | 162.396 µL | 324.791 µL | 1.624 mL | 3.248 mL |
10 mM | 16.24 µL | 81.198 µL | 162.396 µL | 811.978 µL | 1.624 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 544 |
Extinction coefficient (cm -1 M -1) | 740001 |
Excitation (nm) | 545 |
Emission (nm) | 567 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
6-FAM Azide | 493 | 517 | 83000 | 0.178 |
6-HEX azide | 533 | 559 | 73000 | 0.15 |
6-TET azide | 521 | 542 | 73000 | 0.13 |
6-JOE azide | 520 | 545 | 750001 | - |
6-TAMRA azide | 552 | 578 | 90000 | 0.178 |
6-ROX azide | 578 | 604 | 82000 | 0.168 |
6-NED, SE [6-NED NHS ester] | 545 | 567 | 740001 | - |
6-OG488 Azide | 498 | 526 | 76000 | 0.12 |
6-VIC Azide | 526 | 543 | - | - |
References
View all 22 references: Citation Explorer
Assessment of genetic diversity and bottleneck in Purnathadi buffaloes using short tandem repeat markers.
Authors: Ali, S Sajid and Kuralkar, S V and Das, Ramendra and Raina, Varinder and Kataria, R S and Vohra, Vikas
Journal: Animal biotechnology (2020): 1-12
Authors: Ali, S Sajid and Kuralkar, S V and Das, Ramendra and Raina, Varinder and Kataria, R S and Vohra, Vikas
Journal: Animal biotechnology (2020): 1-12
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
HLA Class III: A susceptibility region to systemic lupus erythematosus in Tunisian population.
Authors: Hachicha, Hend and Mahfoudh, Nadia and Fourati, Hajer and Elloumi, Nesrine and Marzouk, Sameh and Feki, Sawsan and Fakhfakh, Raouia and Frikha, Faten and Ayadi, Abir and Maatoug, Amira and Gaddour, Lilia and Hakim, Feiza and Bahloul, Zouheir and Makni, Hafedh and Masmoudi, Hatem and Kammoun, Arwa
Journal: PloS one (2018): e0198549
Authors: Hachicha, Hend and Mahfoudh, Nadia and Fourati, Hajer and Elloumi, Nesrine and Marzouk, Sameh and Feki, Sawsan and Fakhfakh, Raouia and Frikha, Faten and Ayadi, Abir and Maatoug, Amira and Gaddour, Lilia and Hakim, Feiza and Bahloul, Zouheir and Makni, Hafedh and Masmoudi, Hatem and Kammoun, Arwa
Journal: PloS one (2018): e0198549
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
Efficient multiplex simple sequence repeat genotyping of the oomycete plant pathogen Phytophthora infestans.
Authors: Li, Ying and Cooke, David E L and Jacobsen, Evert and van der Lee, Theo
Journal: Journal of microbiological methods (2013): 316-22
Authors: Li, Ying and Cooke, David E L and Jacobsen, Evert and van der Lee, Theo
Journal: Journal of microbiological methods (2013): 316-22
[Genetic polymorphisms of 15 X-STR loci in Shandong Han population].
Authors: Ma, Teng and Xu, Jing and Yang, Ya-Jun and Sun, Kuan and Xue, Fu-Zhong and Jin, Li and Li, Shi-Lin
Journal: Fa yi xue za zhi (2013): 202-5, 208
Authors: Ma, Teng and Xu, Jing and Yang, Ya-Jun and Sun, Kuan and Xue, Fu-Zhong and Jin, Li and Li, Shi-Lin
Journal: Fa yi xue za zhi (2013): 202-5, 208
A new molecular approach to help conclude drowning as a cause of death: simultaneous detection of eight bacterioplankton species using real-time PCR assays with TaqMan probes.
Authors: Uchiyama, Taketo and Kakizaki, Eiji and Kozawa, Shuji and Nishida, Sho and Imamura, Nahoko and Yukawa, Nobuhiro
Journal: Forensic science international (2012): 11-26
Authors: Uchiyama, Taketo and Kakizaki, Eiji and Kozawa, Shuji and Nishida, Sho and Imamura, Nahoko and Yukawa, Nobuhiro
Journal: Forensic science international (2012): 11-26
A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables.
Authors: Elizaquível, Patricia and Aznar, Rosa
Journal: Food microbiology (2008): 705-13
Authors: Elizaquível, Patricia and Aznar, Rosa
Journal: Food microbiology (2008): 705-13
Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36