6-OG488 Azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	637.68
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.31
Correction Factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	76000
Excitation (nm)	498
Emission (nm)	526

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

637.68

Correction Factor (260 nm)

0.31

Correction Factor (280 nm)

0.12

Extinction coefficient (cm ^-1 M ^-1)

76000

Excitation (nm)

498

Emission (nm)

526

6-OG488 azide has the same chemical structure to that of Oregon Green™ 488 azide. It is a convenient tool to introduce the fluorophore of Oregon Green to a biological molecule that contains an alkyne group. It is reactive with terminal alkynes via a copper-catalyzed click reaction. In particular, 6-OG488 azide can be used as a bio-orthogonal or biologically unique hapten for the biological applications requiring signal amplification. Oregon Green is a bright and photostable alternative to fluorescein for use in flow cytometry, fluorescence microscopy and high content analysis of cells. Oregon Green™ is the trademark of ThermoFisher.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-OG488 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	156.818 µL	784.092 µL	1.568 mL	7.841 mL	15.682 mL
5 mM	31.364 µL	156.818 µL	313.637 µL	1.568 mL	3.136 mL
10 mM	15.682 µL	78.409 µL	156.818 µL	784.092 µL	1.568 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.31
Correction Factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	76000
Excitation (nm)	498
Emission (nm)	526

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM Azide	493	517	83000	0.178
6-HEX azide	533	559	73000	0.15
6-TET azide	521	542	73000	0.13
6-JOE azide	520	545	75000¹	-
6-TAMRA azide	552	578	90000	0.178
6-ROX azide	578	604	82000	0.168
6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer]	498	526	76000	0.12
6-NED azide	545	567	74000¹	-
6-VIC Azide	526	543	-	-

Images

Figure 1. Chemical structure for 6-OG488 Azide.

References

View all 50 references: Citation Explorer

Photoactivatable Fluorescent Tags for Dual-Modality Positron Emission Tomography Optical Imaging.
Authors: Guillou, Amaury and Nisli, Eda and Klingler, Simon and Linden, Anthony and Holland, Jason P
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Selective Mitochondrial Protein Labeling Enabled by Biocompatible Photocatalytic Reactions inside Live Cells.
Authors: Wang, Haoyan and Zhang, Yixin and Zeng, Kaixing and Qiang, Jiali and Cao, Ye and Li, Yunxia and Fang, Yanshan and Zhang, Yaoyang and Chen, Yiyun
Journal: JACS Au (2021): 1066-1075

Cell-surface Labeling via Bioorthogonal Host-Guest Chemistry.
Authors: Kataki-Anastasakou, Anna and Hernandez, Selena and Sletten, Ellen M
Journal: ACS chemical biology (2021): 2124-2129

Interfacial dilatational and emulsifying properties of ultrasound-treated pea protein.
Authors: Sha, Lei and Koosis, Aeneas O and Wang, Qingling and True, Alma D and Xiong, Youling L
Journal: Food chemistry (2021): 129271

Design and Synthesis of Fluorophore-Tagged Disparlure Enantiomers to Study Pheromone Enantiomer Discrimination in the Pheromone-Binding Proteins from the Gypsy Moth, Lymantria dispar.
Authors: Pinnelli, Govardhana R and Plettner, Erika
Journal: Journal of chemical ecology (2021)

Glucose-Sensitive Core-Cross-Linked Nanoparticles Constructed with Polyphosphoester Diblock Copolymer for Controlling Insulin Delivery.
Authors: Li, Hongping and Zhou, Ru and He, Jinlin and Zhang, Mingzu and Liu, Jian and Sun, Xingwei and Ni, Peihong
Journal: Bioconjugate chemistry (2021): 2095-2107

Eosin Y as a high-efficient photooxidase mimic for colorimetric detection of sodium azide.
Authors: Wang, Junren and Yu, Haili and He, Yi
Journal: Analytical and bioanalytical chemistry (2020): 7595-7602

Click Chemistry on the Surface of Ultrasmall Gold Nanoparticles (2 nm) for Covalent Ligand Attachment Followed by NMR Spectroscopy.
Authors: van der Meer, Selina Beatrice and Loza, Kateryna and Wey, Karolin and Heggen, Marc and Beuck, Christine and Bayer, Peter and Epple, Matthias
Journal: Langmuir : the ACS journal of surfaces and colloids (2019): 7191-7204

An ultrasensitive fluorescent aptasensor based on truncated aptamer and AGET ATRP for the detection of bisphenol A.
Authors: Guo, Zhuangzhuang and Tang, Jinfa and Li, Manman and Liu, Yanju and Yang, Huaixia and Kong, Jinming
Journal: Analytical and bioanalytical chemistry (2019): 7807-7815

Fluorescent Patterns by Selective Grafting of a Telechelic Polymer.
Authors: Kopeć, Maciej and Tas, Sinem and Cirelli, Marco and van der Pol, Rianne and de Vries, Ilse and Vancso, G Julius and de Beer, Sissi
Journal: ACS applied polymer materials (2019): 136-140