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6-OG488 Azide

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Physical properties
Molecular weight637.68
Spectral properties
Correction Factor (260 nm)0.31
Correction Factor (280 nm)0.12
Extinction coefficient (cm -1 M -1)76000
Excitation (nm)498
Emission (nm)526
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Fluoresceins
Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
6-OG488 azide has the same chemical structure to that of Oregon Green™ 488 azide. It is a convenient tool to introduce the fluorophore of Oregon Green to a biological molecule that contains an alkyne group. It is reactive with terminal alkynes via a copper-catalyzed click reaction. In particular, 6-OG488 azide can be used as a bio-orthogonal or biologically unique hapten for the biological applications requiring signal amplification. Oregon Green is a bright and photostable alternative to fluorescein for use in flow cytometry, fluorescence microscopy and high content analysis of cells. Oregon Green™ is the trademark of ThermoFisher.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-OG488 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM156.818 µL784.092 µL1.568 mL7.841 mL15.682 mL
5 mM31.364 µL156.818 µL313.637 µL1.568 mL3.136 mL
10 mM15.682 µL78.409 µL156.818 µL784.092 µL1.568 mL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.31
Correction Factor (280 nm)0.12
Extinction coefficient (cm -1 M -1)76000
Excitation (nm)498
Emission (nm)526

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-HEX azide533559730000.15
6-TET azide521542730000.13
6-JOE azide520545750001-
6-TAMRA azide552578900000.178
6-ROX azide578604820000.168
6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer]498526760000.12
6-NED azide545567740001-
6-VIC Azide526543--



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