6-TET azide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 582.18 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.13 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 521 |
Emission (nm) | 542 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
6-TET Acid |
6-TET alkyne |
Overview | ![]() ![]() |
See also: Digital PCR, Fluoresceins, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
Molecular weight 582.18 | Correction Factor (280 nm) 0.13 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 521 | Emission (nm) 542 |
6-TET is widely used in nucleic acid sequencing and related research. 6-TET azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 171.768 µL | 858.841 µL | 1.718 mL | 8.588 mL | 17.177 mL |
5 mM | 34.354 µL | 171.768 µL | 343.536 µL | 1.718 mL | 3.435 mL |
10 mM | 17.177 µL | 85.884 µL | 171.768 µL | 858.841 µL | 1.718 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.13 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 521 |
Emission (nm) | 542 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
6-FAM Azide | 493 | 517 | 83000 | 0.178 |
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester] | 521 | 542 | 73000 | 0.13 |
6-HEX azide | 533 | 559 | 73000 | 0.15 |
6-JOE azide | 520 | 545 | 750001 | - |
6-TAMRA azide | 552 | 578 | 90000 | 0.178 |
6-ROX azide | 578 | 604 | 82000 | 0.168 |
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6* | 521 | 542 | 73000 | 0.13 |
6-NED azide | 545 | 567 | 740001 | - |
6-OG488 Azide | 498 | 526 | 76000 | 0.12 |
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Citations
View all 1 citations: Citation Explorer
Involvement of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) in mPR$\alpha$ (PAQR7)-mediated progesterone induction of vascular smooth muscle relaxation
Authors: Pang, Yefei and Thomas, Peter
Journal: American Journal of Physiology-Endocrinology and Metabolism (2021): E453--E466
Authors: Pang, Yefei and Thomas, Peter
Journal: American Journal of Physiology-Endocrinology and Metabolism (2021): E453--E466
References
View all 12 references: Citation Explorer
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Journal: Mol Vis (2006): 1098
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Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
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Authors: Iwamoto T, Sonobe T, Hayashi K.
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Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
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Journal: Nucleic Acids Res (2002): e91
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Journal: Biochemistry (2001): 2484
Strand-separating activity of hepatitis C virus helicase in the absence of ATP
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Authors: Porter DJ, Preugschat F.
Journal: Biochemistry (2000): 5166
Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples
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Journal: J Clin Microbiol (1999): 1941
Authors: Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L.
Journal: J Clin Microbiol (1999): 1941
Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA
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Journal: Biochemistry (1998): 11692
Authors: Gumbs OH, Shaner SL.
Journal: Biochemistry (1998): 11692