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6-TET azide

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Physical properties
Molecular weight582.18
Spectral properties
Correction Factor (280 nm)0.13
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)521
Emission (nm)542
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
6-TET Acid
6-TET alkyne


Molecular weight
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
6-TET is widely used in nucleic acid sequencing and related research. 6-TET azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM171.768 µL858.841 µL1.718 mL8.588 mL17.177 mL
5 mM34.354 µL171.768 µL343.536 µL1.718 mL3.435 mL
10 mM17.177 µL85.884 µL171.768 µL858.841 µL1.718 mL

Molarity calculator

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Spectral properties

Correction Factor (280 nm)0.13
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)521
Emission (nm)542

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester] 521542730000.13
6-HEX azide533559730000.15
6-JOE azide520545750001-
6-TAMRA azide552578900000.178
6-ROX azide578604820000.168
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*521542730000.13
6-NED azide545567740001-
6-OG488 Azide498526760000.12
6-VIC Azide526543--
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Journal: American Journal of Physiology-Endocrinology and Metabolism (2021): E453--E466


View all 12 references: Citation Explorer
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Journal: Mol Vis (2006): 1098
Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
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Journal: Plant J (2004): 947
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
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Journal: J Clin Microbiol (2003): 4016
Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
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Journal: Nucleic Acids Res (2002): e91
Novel algorithm identifies species in a polymycobacterial sample by fluorescence capillary electrophoresis-based single-strand conformation polymorphism analysis
Authors: Iwamoto T, Sonobe T, Hayashi K.
Journal: J Clin Microbiol (2002): 4705
Binding and recognition of GATATC target sequences by the EcoRV restriction endonuclease: a study using fluorescent oligonucleotides and fluorescence polarization
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Journal: Biochemistry (2001): 2484
Strand-separating activity of hepatitis C virus helicase in the absence of ATP
Authors: Porter DJ, Preugschat F.
Journal: Biochemistry (2000): 5166
Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples
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Journal: J Clin Microbiol (1999): 1941
The DNA binding characteristics of the trimeric EcoKI methyltransferase and its partially assembled dimeric form determined by fluorescence polarisation and DNA footprinting
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Journal: J Mol Biol (1998): 947