6-TET azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	582.18
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.13
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	521
Emission (nm)	542

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Molecular weight

582.18

Correction Factor (280 nm)

0.13

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

521

Emission (nm)

542

6-TET is widely used in nucleic acid sequencing and related research. 6-TET azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	171.768 µL	858.841 µL	1.718 mL	8.588 mL	17.177 mL
5 mM	34.354 µL	171.768 µL	343.536 µL	1.718 mL	3.435 mL
10 mM	17.177 µL	85.884 µL	171.768 µL	858.841 µL	1.718 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.13
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	521
Emission (nm)	542

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM Azide	493	517	83000	0.178
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]	521	542	73000	0.13
6-HEX azide	533	559	73000	0.15
6-JOE azide	520	545	75000¹	-
6-TAMRA azide	552	578	90000	0.178
6-ROX azide	578	604	82000	0.168
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] CAS#: 877049-90-6	521	542	73000	0.13
6-NED azide	545	567	74000¹	-
6-OG488 Azide	498	526	76000	0.12
6-VIC Azide	526	543	-	-
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Images

Figure 1. Chemical structure for 6-TET azide

Citations

View all 1 citations: Citation Explorer

Involvement of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) in mPR$\alpha$ (PAQR7)-mediated progesterone induction of vascular smooth muscle relaxation
Authors: Pang, Yefei and Thomas, Peter
Journal: American Journal of Physiology-Endocrinology and Metabolism (2021): E453--E466

References

View all 12 references: Citation Explorer

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Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098

Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947

Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61

Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016

Novel algorithm identifies species in a polymycobacterial sample by fluorescence capillary electrophoresis-based single-strand conformation polymorphism analysis
Authors: Iwamoto T, Sonobe T, Hayashi K.
Journal: J Clin Microbiol (2002): 4705

Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91

Binding and recognition of GATATC target sequences by the EcoRV restriction endonuclease: a study using fluorescent oligonucleotides and fluorescence polarization
Authors: Reid SL, Parry D, Liu HH, Connolly BA.
Journal: Biochemistry (2001): 2484

Strand-separating activity of hepatitis C virus helicase in the absence of ATP
Authors: Porter DJ, Preugschat F.
Journal: Biochemistry (2000): 5166

Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples
Authors: Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L.
Journal: J Clin Microbiol (1999): 1941

Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA
Authors: Gumbs OH, Shaner SL.
Journal: Biochemistry (1998): 11692