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AAT Bioquest

6-TET azide

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight582.18
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.13
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)521
Emission (nm)542
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Alternative formats
6-TET alkyne

OverviewpdfSDSpdfProtocol


Molecular weight
582.18
Correction Factor (280 nm)
0.13
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)
521
Emission (nm)
542
6-TET is widely used in nucleic acid sequencing and related research. 6-TET azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM171.768 µL858.841 µL1.718 mL8.588 mL17.177 mL
5 mM34.354 µL171.768 µL343.536 µL1.718 mL3.435 mL
10 mM17.177 µL85.884 µL171.768 µL858.841 µL1.718 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.13
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)521
Emission (nm)542

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester] 521542730000.13
6-HEX azide533559730000.15
6-JOE azide520545750001-
6-TAMRA azide552578900000.178
6-ROX azide578604820000.168
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*521542730000.13
6-NED azide545567740001-
6-OG488 Azide498526760000.12

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Citations


View all 1 citations: Citation Explorer
Involvement of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) in mPR$\alpha$ (PAQR7)-mediated progesterone induction of vascular smooth muscle relaxation
Authors: Pang, Yefei and Thomas, Peter
Journal: American Journal of Physiology-Endocrinology and Metabolism (2021): E453--E466

References


View all 12 references: Citation Explorer
Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098
Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947
Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016
Novel algorithm identifies species in a polymycobacterial sample by fluorescence capillary electrophoresis-based single-strand conformation polymorphism analysis
Authors: Iwamoto T, Sonobe T, Hayashi K.
Journal: J Clin Microbiol (2002): 4705
Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91
Binding and recognition of GATATC target sequences by the EcoRV restriction endonuclease: a study using fluorescent oligonucleotides and fluorescence polarization
Authors: Reid SL, Parry D, Liu HH, Connolly BA.
Journal: Biochemistry (2001): 2484
Strand-separating activity of hepatitis C virus helicase in the absence of ATP
Authors: Porter DJ, Preugschat F.
Journal: Biochemistry (2000): 5166
Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples
Authors: Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L.
Journal: J Clin Microbiol (1999): 1941
Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA
Authors: Gumbs OH, Shaner SL.
Journal: Biochemistry (1998): 11692