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6-ROX azide

6-ROX azide is an excellent alkyne-reactive fluorescent reagent for labeling alkyne-containing biological molecules through the well known click chemistry. It has the spectral properties identical to those of other 6-ROX conjugates, thus all the instrument settings of the 6-ROX conjugates would be applicable to the applications of 6-ROX azide conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROX azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM165.926 µL829.628 µL1.659 mL8.296 mL16.593 mL
5 mM33.185 µL165.926 µL331.851 µL1.659 mL3.319 mL
10 mM16.593 µL82.963 µL165.926 µL829.628 µL1.659 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-HEX azide533559730000.15
6-TET azide521542730000.13
6-JOE azide520545750001-
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*578604820000.168
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*578604820000.168
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*578604820000.168
6-TAMRA azide552578900000.178
6-NED azide545567740001-
6-OG488 Azide498526760000.12
6-VIC Azide526543--
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References

View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Page updated on October 12, 2024

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Physical properties

Molecular weight

602.68

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.168

Extinction coefficient (cm -1 M -1)

82000

Excitation (nm)

578

Emission (nm)

604

Quantum yield

0.941

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501