6-HEX azide

6-HEX is widely used in nucleic acid sequencing and related research. 6-HEX azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
240	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	153.593 µL	767.967 µL	1.536 mL	7.68 mL	15.359 mL
5 mM	30.719 µL	153.593 µL	307.187 µL	1.536 mL	3.072 mL
10 mM	15.359 µL	76.797 µL	153.593 µL	767.967 µL	1.536 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	651.07 g/mol	=	mL	x	mM	=	mmol

References

View all 12 references: Citation Explorer

Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis

Authors:

Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.

Journal:

Mol Vis (2006): 1098

Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues

Authors:

Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.

Journal:

Plant J (2004): 947

Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species

Authors:

Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.

Journal:

J Clin Microbiol (2003): 4016

Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography

Authors:

Fountain KJ, Gilar M, Budman Y, Gebler JC.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61

Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection

Authors:

Simeonov A, Nikiforov TT.

Journal:

Nucleic Acids Res (2002): e91

Physical properties

Molecular weight	651.07
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	533
Emission (nm)	559

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 10, 2025