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6-HEX azide
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Application notes
6-HEX is widely used in nucleic acid sequencing and related research. 6-HEX azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 153.593 µL | 767.967 µL | 1.536 mL | 7.68 mL | 15.359 mL |
| 5 mM | 30.719 µL | 153.593 µL | 307.187 µL | 1.536 mL | 3.072 mL |
| 10 mM | 15.359 µL | 76.797 µL | 153.593 µL | 767.967 µL | 1.536 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-FAM Azide | 493 | 517 | 83000 | 0.178 |
| 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* | 533 | 559 | 73000 | 0.15 |
| 6-TET azide | 521 | 542 | 73000 | 0.13 |
| 6-JOE azide | 520 | 545 | 750001 | - |
| 6-TAMRA azide | 552 | 578 | 90000 | 0.178 |
| 6-ROX azide | 578 | 604 | 82000 | 0.168 |
| 6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite] | 533 | 559 | 73000 | 0.15 |
| 6-NED azide | 545 | 567 | 740001 | - |
| 6-OG488 Azide | 498 | 526 | 76000 | 0.12 |
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References
View all 12 references: Citation Explorer
Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098
Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016
Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91