6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer*

6-HEX, SE

6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester; Single Isomer

6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research.

Catalog	Size	Price	Quantity
202	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	147.046 µL	735.229 µL	1.47 mL	7.352 mL	14.705 mL
5 mM	29.409 µL	147.046 µL	294.092 µL	1.47 mL	2.941 mL
10 mM	14.705 µL	73.523 µL	147.046 µL	735.229 µL	1.47 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	680.06 g/mol	=	mL	x	mM	=	mmol

References

View all 12 references: Citation Explorer

Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis

Authors:

Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.

Journal:

Mol Vis (2006): 1098

Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues

Authors:

Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.

Journal:

Plant J (2004): 947

Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species

Authors:

Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.

Journal:

J Clin Microbiol (2003): 4016

Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography

Authors:

Fountain KJ, Gilar M, Budman Y, Gebler JC.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61

Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection

Authors:

Simeonov A, Nikiforov TT.

Journal:

Nucleic Acids Res (2002): e91

Physical properties

Molecular weight	680.06
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	533
Emission (nm)	559

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	2129651-79-0

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on October 27, 2025