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6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8*

6-FAM, SE is the amine-reactive succinimidyl ester of single isomer 6-FAM acid. It is one of the most popular green fluorescent reagents used for labeling nucleotides and nucleic acids. Compared to 5-FAM, 6-FAM is less often used to prepare small molecules.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM211.242 µL1.056 mL2.112 mL10.562 mL21.124 mL
5 mM42.248 µL211.242 µL422.485 µL2.112 mL4.225 mL
10 mM21.124 µL105.621 µL211.242 µL1.056 mL2.112 mL

Molarity calculator

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Spectrum

Citations

View all 3 citations: Citation Explorer
Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 137 references: Citation Explorer
Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80
Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165
Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619
Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379
Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262
Page updated on November 3, 2024

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Physical properties

Molecular weight

473.39

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm -1 M -1)

83000

Excitation (nm)

493

Emission (nm)

517

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

92557-81-8