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6-VIC Azide

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Physical properties
Molecular weight623.83
SolventDMSO
Spectral properties
Excitation (nm)526
Emission (nm)543
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
6-VIC Alkyne

OverviewpdfSDSpdfProtocol


Molecular weight
623.83
Excitation (nm)
526
Emission (nm)
543
6-VIC is a popular fluorophore that is widely used in nucleic acid sequencing and related research. It is also often used for labeling oligos. 6-VIC azide is a convenient building block that is used to label a biomolecule that contains an alkyne group, such as alkyne-modified oligos.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-VIC Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM160.3 µL801.5 µL1.603 mL8.015 mL16.03 mL
5 mM32.06 µL160.3 µL320.6 µL1.603 mL3.206 mL
10 mM16.03 µL80.15 µL160.3 µL801.5 µL1.603 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)526
Emission (nm)543

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-HEX azide533559730000.15
6-TET azide521542730000.13
6-JOE azide520545750001-
6-TAMRA azide552578900000.178
6-ROX azide578604820000.168
6-VIC, SE [6-VIC NHS ester]526543--
6-NED azide545567740001-
6-OG488 Azide498526760000.12

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References


View all 50 references: Citation Explorer
Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex.
Authors: Brush, George S
Journal: PloS one (2022): e0279423
DNA sequencing by Förster resonant energy transfer.
Authors: Fotouhi, Bashir and Faramarzi, Vahid and Ahmadi, Vahid
Journal: Optics express (2022): 21854-21865
Cultivating DNA Sequencing Technology After the Human Genome Project.
Authors: Schloss, Jeffery A and Gibbs, Richard A and Makhijani, Vinod B and Marziali, Andre
Journal: Annual review of genomics and human genetics (2020): 117-138
High Throughput Sediment DNA Sequencing Reveals Azo Dye Degrading Bacteria Inhabit Nearshore Sediments.
Authors: Zhuang, Mei and Sanganyado, Edmond and Xu, Liang and Zhu, Jianming and Li, Ping and Liu, Wenhua
Journal: Microorganisms (2020)
Propidium monoazide conventional PCR and DNA sequencing: detection of negative culture bacterial pathogens causing subclinical mastitis.
Authors: Farhan, M G and Abd El-Hamid, M I and Hassan, M N
Journal: Journal of applied microbiology (2020): 1595-1605
Thermococcus sp. 9°N DNA polymerase exhibits 3'-esterase activity that can be harnessed for DNA sequencing.
Authors: LinWu, Shiuan-Woei and Tu, Yu-Hsuan and Tsai, Ting-Yueh and Maestre-Reyna, Manuel and Liu, Mu-Sen and Wu, Wen-Jin and Huang, Jyun-Yuan and Chi, Hung-Wen and Chang, Wei-Hsin and Chiou, Chung-Fan and Wang, Andrew H-J and Lee, Johnsee and Tsai, Ming-Daw
Journal: Communications biology (2019): 224
Highly accurate fluorogenic DNA sequencing with information theory-based error correction.
Authors: Chen, Zitian and Zhou, Wenxiong and Qiao, Shuo and Kang, Li and Duan, Haifeng and Xie, X Sunney and Huang, Yanyi
Journal: Nature biotechnology (2017): 1170-1178
Data on single-step purification method for dye-labeled DNA sequencing.
Authors: Fujikura, Kohei
Journal: Data in brief (2016): 873-6
Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing.
Authors: Tan, Lianjiang and Liu, Yazhi and Li, Xiaowei and Wu, Xin-Yan and Gong, Bing and Shen, Yu-Mei and Shao, Zhifeng
Journal: Chemical communications (Cambridge, England) (2016): 2549-52
No-wash ethanol precipitation of dye-labeled reaction products improves DNA sequencing reads.
Authors: Fujikura, Kohei
Journal: Analytical biochemistry (2015): 39-41