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6-VIC Azide

6-VIC is a popular fluorophore that is widely used in nucleic acid sequencing and related research. It is also often used for labeling oligos. 6-VIC azide is a convenient building block that is used to label a biomolecule that contains an alkyne group, such as alkyne-modified oligos.
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Catalog Number219
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Physical properties
Molecular weight623.83
SolventDMSO
Spectral properties
Excitation (nm)526
Emission (nm)543
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Spectrum
Product family
NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-HEX azide533559730000.15
6-TET azide521542730000.13
6-JOE azide520545750001-
6-TAMRA azide552578900000.178
6-ROX azide578604820000.168
6-VIC, SE [6-VIC NHS ester]526543--
6-NED azide545567740001-
6-OG488 Azide498526760000.12
6-VIC phosphoramidite526543--
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References
View all 50 references: Citation Explorer
Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex.
Authors: Brush, George S
Journal: PloS one (2022): e0279423
DNA sequencing by Förster resonant energy transfer.
Authors: Fotouhi, Bashir and Faramarzi, Vahid and Ahmadi, Vahid
Journal: Optics express (2022): 21854-21865
High Throughput Sediment DNA Sequencing Reveals Azo Dye Degrading Bacteria Inhabit Nearshore Sediments.
Authors: Zhuang, Mei and Sanganyado, Edmond and Xu, Liang and Zhu, Jianming and Li, Ping and Liu, Wenhua
Journal: Microorganisms (2020)
Propidium monoazide conventional PCR and DNA sequencing: detection of negative culture bacterial pathogens causing subclinical mastitis.
Authors: Farhan, M G and Abd El-Hamid, M I and Hassan, M N
Journal: Journal of applied microbiology (2020): 1595-1605
Cultivating DNA Sequencing Technology After the Human Genome Project.
Authors: Schloss, Jeffery A and Gibbs, Richard A and Makhijani, Vinod B and Marziali, Andre
Journal: Annual review of genomics and human genetics (2020): 117-138