6-VIC Azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	623.83
Solvent	DMSO

Spectral properties

Excitation (nm)	526
Emission (nm)	543

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-VIC Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	160.3 µL	801.5 µL	1.603 mL	8.015 mL	16.03 mL
5 mM	32.06 µL	160.3 µL	320.6 µL	1.603 mL	3.206 mL
10 mM	16.03 µL	80.15 µL	160.3 µL	801.5 µL	1.603 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	526
Emission (nm)	543

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM Azide	493	517	83000	0.178
6-HEX azide	533	559	73000	0.15
6-TET azide	521	542	73000	0.13
6-JOE azide	520	545	75000¹	-
6-TAMRA azide	552	578	90000	0.178
6-ROX azide	578	604	82000	0.168
6-VIC, SE [6-VIC NHS ester]	526	543	-	-
6-NED azide	545	567	74000¹	-
6-OG488 Azide	498	526	76000	0.12
6-VIC phosphoramidite	526	543	-	-
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Images

Figure 1. 6-VIC azide is a fluorescent compound that contains an azido group (-N3) used in a variety of DNA sequencing and PCR applications. It is often used in combination with a complementary molecule bearing an alkyne group, such as propargylamine or dibenzylcyclooctyne (DBCO), in a reaction known as the Huisgen cycloaddition or "click" reaction. This reaction forms a stable triazole linkage between the azido and alkyne groups, allowing for the covalent attachment of a variety of biomolecules, drugs, or imaging agents to a target molecule or surface.

References

View all 50 references: Citation Explorer

Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex.
Authors: Brush, George S
Journal: PloS one (2022): e0279423

DNA sequencing by Förster resonant energy transfer.
Authors: Fotouhi, Bashir and Faramarzi, Vahid and Ahmadi, Vahid
Journal: Optics express (2022): 21854-21865

Cultivating DNA Sequencing Technology After the Human Genome Project.
Authors: Schloss, Jeffery A and Gibbs, Richard A and Makhijani, Vinod B and Marziali, Andre
Journal: Annual review of genomics and human genetics (2020): 117-138

High Throughput Sediment DNA Sequencing Reveals Azo Dye Degrading Bacteria Inhabit Nearshore Sediments.
Authors: Zhuang, Mei and Sanganyado, Edmond and Xu, Liang and Zhu, Jianming and Li, Ping and Liu, Wenhua
Journal: Microorganisms (2020)

Propidium monoazide conventional PCR and DNA sequencing: detection of negative culture bacterial pathogens causing subclinical mastitis.
Authors: Farhan, M G and Abd El-Hamid, M I and Hassan, M N
Journal: Journal of applied microbiology (2020): 1595-1605

Thermococcus sp. 9°N DNA polymerase exhibits 3'-esterase activity that can be harnessed for DNA sequencing.
Authors: LinWu, Shiuan-Woei and Tu, Yu-Hsuan and Tsai, Ting-Yueh and Maestre-Reyna, Manuel and Liu, Mu-Sen and Wu, Wen-Jin and Huang, Jyun-Yuan and Chi, Hung-Wen and Chang, Wei-Hsin and Chiou, Chung-Fan and Wang, Andrew H-J and Lee, Johnsee and Tsai, Ming-Daw
Journal: Communications biology (2019): 224

Highly accurate fluorogenic DNA sequencing with information theory-based error correction.
Authors: Chen, Zitian and Zhou, Wenxiong and Qiao, Shuo and Kang, Li and Duan, Haifeng and Xie, X Sunney and Huang, Yanyi
Journal: Nature biotechnology (2017): 1170-1178

Data on single-step purification method for dye-labeled DNA sequencing.
Authors: Fujikura, Kohei
Journal: Data in brief (2016): 873-6

Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing.
Authors: Tan, Lianjiang and Liu, Yazhi and Li, Xiaowei and Wu, Xin-Yan and Gong, Bing and Shen, Yu-Mei and Shao, Zhifeng
Journal: Chemical communications (Cambridge, England) (2016): 2549-52

No-wash ethanol precipitation of dye-labeled reaction products improves DNA sequencing reads.
Authors: Fujikura, Kohei
Journal: Analytical biochemistry (2015): 39-41