6-VIC Azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 623.83 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 526 |
| Emission (nm) | 543 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| 6-VIC Alkyne |
| Overview |  SDSProtocol |
Molecular weight 623.83 | Excitation (nm) 526 | Emission (nm) 543 |
6-VIC is a popular fluorophore that is widely used in nucleic acid sequencing and related research. It is also often used for labeling oligos. 6-VIC azide is a convenient building block that is used to label a biomolecule that contains an alkyne group, such as alkyne-modified oligos.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-VIC Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 160.3 µL | 801.5 µL | 1.603 mL | 8.015 mL | 16.03 mL |
| 5 mM | 32.06 µL | 160.3 µL | 320.6 µL | 1.603 mL | 3.206 mL |
| 10 mM | 16.03 µL | 80.15 µL | 160.3 µL | 801.5 µL | 1.603 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-FAM Azide | 493 | 517 | 83000 | 0.178 |
| 6-HEX azide | 533 | 559 | 73000 | 0.15 |
| 6-TET azide | 521 | 542 | 73000 | 0.13 |
| 6-JOE azide | 520 | 545 | 750001 | - |
| 6-TAMRA azide | 552 | 578 | 90000 | 0.178 |
| 6-ROX azide | 578 | 604 | 82000 | 0.168 |
| 6-VIC, SE [6-VIC NHS ester] | 526 | 543 | - | - |
| 6-NED azide | 545 | 567 | 740001 | - |
| 6-OG488 Azide | 498 | 526 | 76000 | 0.12 |
Images
Figure 1. 6-VIC azide is a fluorescent compound that contains an azido group (-N3) used in a variety of DNA sequencing and PCR applications. It is often used in combination with a complementary molecule bearing an alkyne group, such as propargylamine or dibenzylcyclooctyne (DBCO), in a reaction known as the Huisgen cycloaddition or "click" reaction. This reaction forms a stable triazole linkage between the azido and alkyne groups, allowing for the covalent attachment of a variety of biomolecules, drugs, or imaging agents to a target molecule or surface.
References
View all 50 references: Citation Explorer
Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex.
Authors: Brush, George S
Journal: PloS one (2022): e0279423
Authors: Brush, George S
Journal: PloS one (2022): e0279423
DNA sequencing by Förster resonant energy transfer.
Authors: Fotouhi, Bashir and Faramarzi, Vahid and Ahmadi, Vahid
Journal: Optics express (2022): 21854-21865
Authors: Fotouhi, Bashir and Faramarzi, Vahid and Ahmadi, Vahid
Journal: Optics express (2022): 21854-21865
Cultivating DNA Sequencing Technology After the Human Genome Project.
Authors: Schloss, Jeffery A and Gibbs, Richard A and Makhijani, Vinod B and Marziali, Andre
Journal: Annual review of genomics and human genetics (2020): 117-138
Authors: Schloss, Jeffery A and Gibbs, Richard A and Makhijani, Vinod B and Marziali, Andre
Journal: Annual review of genomics and human genetics (2020): 117-138
High Throughput Sediment DNA Sequencing Reveals Azo Dye Degrading Bacteria Inhabit Nearshore Sediments.
Authors: Zhuang, Mei and Sanganyado, Edmond and Xu, Liang and Zhu, Jianming and Li, Ping and Liu, Wenhua
Journal: Microorganisms (2020)
Authors: Zhuang, Mei and Sanganyado, Edmond and Xu, Liang and Zhu, Jianming and Li, Ping and Liu, Wenhua
Journal: Microorganisms (2020)
Propidium monoazide conventional PCR and DNA sequencing: detection of negative culture bacterial pathogens causing subclinical mastitis.
Authors: Farhan, M G and Abd El-Hamid, M I and Hassan, M N
Journal: Journal of applied microbiology (2020): 1595-1605
Authors: Farhan, M G and Abd El-Hamid, M I and Hassan, M N
Journal: Journal of applied microbiology (2020): 1595-1605
Thermococcus sp. 9°N DNA polymerase exhibits 3'-esterase activity that can be harnessed for DNA sequencing.
Authors: LinWu, Shiuan-Woei and Tu, Yu-Hsuan and Tsai, Ting-Yueh and Maestre-Reyna, Manuel and Liu, Mu-Sen and Wu, Wen-Jin and Huang, Jyun-Yuan and Chi, Hung-Wen and Chang, Wei-Hsin and Chiou, Chung-Fan and Wang, Andrew H-J and Lee, Johnsee and Tsai, Ming-Daw
Journal: Communications biology (2019): 224
Authors: LinWu, Shiuan-Woei and Tu, Yu-Hsuan and Tsai, Ting-Yueh and Maestre-Reyna, Manuel and Liu, Mu-Sen and Wu, Wen-Jin and Huang, Jyun-Yuan and Chi, Hung-Wen and Chang, Wei-Hsin and Chiou, Chung-Fan and Wang, Andrew H-J and Lee, Johnsee and Tsai, Ming-Daw
Journal: Communications biology (2019): 224
Highly accurate fluorogenic DNA sequencing with information theory-based error correction.
Authors: Chen, Zitian and Zhou, Wenxiong and Qiao, Shuo and Kang, Li and Duan, Haifeng and Xie, X Sunney and Huang, Yanyi
Journal: Nature biotechnology (2017): 1170-1178
Authors: Chen, Zitian and Zhou, Wenxiong and Qiao, Shuo and Kang, Li and Duan, Haifeng and Xie, X Sunney and Huang, Yanyi
Journal: Nature biotechnology (2017): 1170-1178
Data on single-step purification method for dye-labeled DNA sequencing.
Authors: Fujikura, Kohei
Journal: Data in brief (2016): 873-6
Authors: Fujikura, Kohei
Journal: Data in brief (2016): 873-6
Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing.
Authors: Tan, Lianjiang and Liu, Yazhi and Li, Xiaowei and Wu, Xin-Yan and Gong, Bing and Shen, Yu-Mei and Shao, Zhifeng
Journal: Chemical communications (Cambridge, England) (2016): 2549-52
Authors: Tan, Lianjiang and Liu, Yazhi and Li, Xiaowei and Wu, Xin-Yan and Gong, Bing and Shen, Yu-Mei and Shao, Zhifeng
Journal: Chemical communications (Cambridge, England) (2016): 2549-52
No-wash ethanol precipitation of dye-labeled reaction products improves DNA sequencing reads.
Authors: Fujikura, Kohei
Journal: Analytical biochemistry (2015): 39-41
Authors: Fujikura, Kohei
Journal: Analytical biochemistry (2015): 39-41