Calculator
6-TET Acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 514.09 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.13 |
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 521 |
| Emission (nm) | 542 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| 6-TET azide |
| 6-TET alkyne |
| Overview |  SDSProtocol |
Molecular weight 514.09 | Correction Factor (280 nm) 0.13 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 521 | Emission (nm) 542 |
6-TET is a fluorophore that is widely used in nucleic acid sequencing and related research. 6-TET acid is the most economic form to make a conjugate with amino-containing biomolecules.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET Acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 194.518 µL | 972.592 µL | 1.945 mL | 9.726 mL | 19.452 mL |
| 5 mM | 38.904 µL | 194.518 µL | 389.037 µL | 1.945 mL | 3.89 mL |
| 10 mM | 19.452 µL | 97.259 µL | 194.518 µL | 972.592 µL | 1.945 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.13 |
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 521 |
| Emission (nm) | 542 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester] | 521 | 542 | 73000 | 0.13 |
| 6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6* | 521 | 542 | 73000 | 0.13 |
| 6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer] | 498 | 526 | 76000 | 0.12 |
| 6-ROXtra™ acid | 578 | 595 | - | - |
| 6-JOE acid | 520 | 545 | 750001 | - |
| 6-HEX acid | 533 | 559 | 73000 | 0.15 |
Images
Figure 1. 6-TET is a fluorophore that is widely used in nucleic acid sequencing and related research. 6-TET acid is the most economic form to make a conjugate with amino-containing biomolecules.
References
View all 17 references: Citation Explorer
Dye-encapsulated Zr-based MOFs composites as a sensitive platform for ratiometric luminescent sensing of antibiotics in water.
Authors: Li, Jingyu and Dai, Yexin and Cui, Jinran and Abrha, Halayit and Kang, Ning and Liu, Xianhua
Journal: Talanta (2023): 123817
Authors: Li, Jingyu and Dai, Yexin and Cui, Jinran and Abrha, Halayit and Kang, Ning and Liu, Xianhua
Journal: Talanta (2023): 123817
Krüppel-like factor 5 is essential for maintenance of barrier function in mouse colon.
Authors: Liu, Yang and Chidgey, Martyn and Yang, Vincent W and Bialkowska, Agnieszka B
Journal: American journal of physiology. Gastrointestinal and liver physiology (2017): G478-G491
Authors: Liu, Yang and Chidgey, Martyn and Yang, Vincent W and Bialkowska, Agnieszka B
Journal: American journal of physiology. Gastrointestinal and liver physiology (2017): G478-G491
Evaluation of Ocular Surface Disease in Asian Patients with Primary Angle Closure.
Authors: Ling, Tan Ee and Othman, Khairuddin and Yan, Ong Poh and Rashid, Rasdi Abdul and Tet, Cheong Min and Yaakob, Azhany and Tajudin, Liza-Sharmini Ahmad
Journal: The open ophthalmology journal (2017): 31-39
Authors: Ling, Tan Ee and Othman, Khairuddin and Yan, Ong Poh and Rashid, Rasdi Abdul and Tet, Cheong Min and Yaakob, Azhany and Tajudin, Liza-Sharmini Ahmad
Journal: The open ophthalmology journal (2017): 31-39
Spatiotemporally Controlled Ablation of Klf5 Results in Dysregulated Epithelial Homeostasis in Adult Mouse Corneas.
Authors: Loughner, Chelsea L and Tiwari, Anil and Kenchegowda, Doreswamy and Swamynathan, Sudha and Swamynathan, Shivalingappa K
Journal: Investigative ophthalmology & visual science (2017): 4683-4693
Authors: Loughner, Chelsea L and Tiwari, Anil and Kenchegowda, Doreswamy and Swamynathan, Sudha and Swamynathan, Shivalingappa K
Journal: Investigative ophthalmology & visual science (2017): 4683-4693
Spatiotemporally Regulated Ablation of Klf4 in Adult Mouse Corneal Epithelial Cells Results in Altered Epithelial Cell Identity and Disrupted Homeostasis.
Authors: Delp, Emili E and Swamynathan, Sudha and Kao, Winston W and Swamynathan, Shivalingappa K
Journal: Investigative ophthalmology & visual science (2015): 3549-58
Authors: Delp, Emili E and Swamynathan, Sudha and Kao, Winston W and Swamynathan, Shivalingappa K
Journal: Investigative ophthalmology & visual science (2015): 3549-58
Nitric oxide directly promotes vascular endothelial insulin transport.
Authors: Wang, Hong and Wang, Aileen X and Aylor, Kevin and Barrett, Eugene J
Journal: Diabetes (2013): 4030-42
Authors: Wang, Hong and Wang, Aileen X and Aylor, Kevin and Barrett, Eugene J
Journal: Diabetes (2013): 4030-42
Subretinal transplantation of rat MSCs and erythropoietin gene modified rat MSCs for protecting and rescuing degenerative retina in rats.
Authors: Guan, Y and Cui, L and Qu, Z and Lu, L and Wang, F and Wu, Y and Zhang, J and Gao, F and Tian, H and Xu, L and Xu, G and Li, W and Jin, Y and Xu, G-T
Journal: Current molecular medicine (2013): 1419-31
Authors: Guan, Y and Cui, L and Qu, Z and Lu, L and Wang, F and Wu, Y and Zhang, J and Gao, F and Tian, H and Xu, L and Xu, G and Li, W and Jin, Y and Xu, G-T
Journal: Current molecular medicine (2013): 1419-31
Synergistic antiangiogenic activity of tetrandrine combined with Endostar on the human umbilical vein endothelial cell model.
Authors: Qian, Xiaoping and Yan, Bo and Zhou, Xuefei and Xie, Li and Wei, Jia and Li, Rutian and Yu, Lixia and Liu, Baorui
Journal: Cancer biotherapy & radiopharmaceuticals (2013): 385-90
Authors: Qian, Xiaoping and Yan, Bo and Zhou, Xuefei and Xie, Li and Wei, Jia and Li, Rutian and Yu, Lixia and Liu, Baorui
Journal: Cancer biotherapy & radiopharmaceuticals (2013): 385-90
Increased survival is a selective feature of human circulating antigen-induced plasma cells synthesizing high-affinity antibodies.
Authors: González-García, Inés and Rodríguez-Bayona, Beatriz and Mora-López, Francisco and Campos-Caro, Antonio and Brieva, José A
Journal: Blood (2008): 741-9
Authors: González-García, Inés and Rodríguez-Bayona, Beatriz and Mora-López, Francisco and Campos-Caro, Antonio and Brieva, José A
Journal: Blood (2008): 741-9
Neuronal affinity of a C7C loop peptide identified through phage display.
Authors: Federici, Thais and Liu, James K and Teng, Qingshan and Garrity-Moses, Mary and Yang, Jun and Boulis, Nicholas M
Journal: Journal of drug targeting (2006): 263-71
Authors: Federici, Thais and Liu, James K and Teng, Qingshan and Garrity-Moses, Mary and Yang, Jun and Boulis, Nicholas M
Journal: Journal of drug targeting (2006): 263-71