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6-JOE alkyne

6-JOE is widely used in nucleic acid sequencing and related research. 6-JOE alkyne provides a convenient method for labeling azide-modified oligos throught the well-known click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-JOE alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM184.393 µL921.965 µL1.844 mL9.22 mL18.439 mL
5 mM36.879 µL184.393 µL368.786 µL1.844 mL3.688 mL
10 mM18.439 µL92.196 µL184.393 µL921.965 µL1.844 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Alkyne493517830000.178
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*520545750001-
6-HEX alkyne533559730000.15
6-TET alkyne521542730000.13
6-TAMRA alkyne552578900000.178
6-ROX alkyne578604820000.168
6-JOE Phosphoramidite520545750001-
6-NED alkyne545567740001-
6-VIC Alkyne526543--

References

View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058
Page updated on October 22, 2024

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Physical properties

Molecular weight

542.32

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

520

Emission (nm)

545

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Alternative formats