6-ROXtra™ acid
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1034.34 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 578 |
Emission (nm) | 595 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Molecular weight 1034.34 | Excitation (nm) 578 | Emission (nm) 595 |
Although ROX dyes have strong fluorescence they are notoriously unstable. Some commercial samples only have a few months’ shelf life. Compared to 6-ROX labeling compounds our 6-ROXtra™ dyes have greatly enhanced stability while they have essentially identical spectral properties to those of 6-ROX when conjugated to a biological substrate (such as oligonucleotides). In addition, our 6-ROXtra™ dyes are much more water-soluble than 6-ROX dyes that are essentially insoluble in water and aqueous buffers. Our preliminary studies indicated that 6-ROXtra-derived oligos are more easily purified than the corresponding 6-ROX oligos in most cases. When conjugated to proteins, the fluorescence of 6-ROX is severely quenched while 6-ROXtra™ still shows strong fluorescence.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROXtra™ acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 96.68 µL | 483.4 µL | 966.8 µL | 4.834 mL | 9.668 mL |
5 mM | 19.336 µL | 96.68 µL | 193.36 µL | 966.8 µL | 1.934 mL |
10 mM | 9.668 µL | 48.34 µL | 96.68 µL | 483.4 µL | 966.8 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
6-ROXtra™ SE *Superior 6-ROX Replacement* | 578 | 595 | - | - |
6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer] | 498 | 526 | 76000 | 0.12 |
6-JOE acid | 520 | 545 | 750001 | - |
6-HEX acid | 533 | 559 | 73000 | 0.15 |
6-TET Acid | 521 | 542 | 73000 | 0.13 |
References
View all 13 references: Citation Explorer
Selenoprotein P inhibits cell proliferation and ROX production in HCC cells.
Authors: Wang, Jianxin and Shen, Pei and Liao, Sha and Duan, Lian and Zhu, Dandan and Chen, Jinling and Chen, Liuting and Sun, Xiaolei and Duan, Yinong
Journal: PloS one (2020): e0236491
Authors: Wang, Jianxin and Shen, Pei and Liao, Sha and Duan, Lian and Zhu, Dandan and Chen, Jinling and Chen, Liuting and Sun, Xiaolei and Duan, Yinong
Journal: PloS one (2020): e0236491
Aligned Expression of IFI16 and STING Genes in RRMS Patients' Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886
SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30
RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.
Authors: Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 27-35
Authors: Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 27-35
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA.
Authors: Farzan, Valentina M and Kvach, Maksim V and Aparin, Ilya O and Kireev, Dmitry E and Prikazchikova, Tatiana A and Ustinov, Alexey V and Shmanai, Vadim V and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Talanta (2019): 226-232
Authors: Farzan, Valentina M and Kvach, Maksim V and Aparin, Ilya O and Kireev, Dmitry E and Prikazchikova, Tatiana A and Ustinov, Alexey V and Shmanai, Vadim V and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Talanta (2019): 226-232
Expression of Plasma hsa-miR122 in HBV-Related Hepatocellular Carcinoma (HCC) in Vietnamese Patients.
Authors: Quoc, Nguyen Bao and Phuong, Nguyen Doan Nguyen and Ngan, Tang Kim and Linh, Nguyen Thi Minh and Cuong, Pham Hung and Chau, Nguyen Ngoc Bao
Journal: MicroRNA (Shariqah, United Arab Emirates) (2018): 92-99
Authors: Quoc, Nguyen Bao and Phuong, Nguyen Doan Nguyen and Ngan, Tang Kim and Linh, Nguyen Thi Minh and Cuong, Pham Hung and Chau, Nguyen Ngoc Bao
Journal: MicroRNA (Shariqah, United Arab Emirates) (2018): 92-99
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
[Short-term Effect of Roxithromycin on Abundance and Diversity of Ammonia-Oxidizing Microorganisms in Activated Sludge].
Authors: Gao, Jing-Feng and Sun, Li-Xin and Fan, Xiao-Yan and Pan, Kai-Ling and Li, Ding-Chang
Journal: Huan jing ke xue= Huanjing kexue (2017): 2961-2971
Authors: Gao, Jing-Feng and Sun, Li-Xin and Fan, Xiao-Yan and Pan, Kai-Ling and Li, Ding-Chang
Journal: Huan jing ke xue= Huanjing kexue (2017): 2961-2971
Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance.
Authors: Jung, Harin and Chung, Pil Joong and Park, Su-Hyun and Redillas, Mark Christian Felipe Reveche and Kim, Youn Shic and Suh, Joo-Won and Kim, Ju-Kon
Journal: Plant biotechnology journal (2017): 1295-1308
Authors: Jung, Harin and Chung, Pil Joong and Park, Su-Hyun and Redillas, Mark Christian Felipe Reveche and Kim, Youn Shic and Suh, Joo-Won and Kim, Ju-Kon
Journal: Plant biotechnology journal (2017): 1295-1308
Application notes
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