6-ROXtra™ acid

Although ROX dyes have strong fluorescence they are notoriously unstable. Some commercial samples only have a few months’ shelf life. Compared to 6-ROX labeling compounds our 6-ROXtra™ dyes have greatly enhanced stability while they have essentially identical spectral properties to those of 6-ROX when conjugated to a biological substrate (such as oligonucleotides). In addition, our 6-ROXtra™ dyes are much more water-soluble than 6-ROX dyes that are essentially insoluble in water and aqueous buffers. Our preliminary studies indicated that 6-ROXtra-derived oligos are more easily purified than the corresponding 6-ROX oligos in most cases. When conjugated to proteins, the fluorescence of 6-ROX is severely quenched while 6-ROXtra™ still shows strong fluorescence.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
386	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROXtra™ acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.68 µL	483.4 µL	966.8 µL	4.834 mL	9.668 mL
5 mM	19.336 µL	96.68 µL	193.36 µL	966.8 µL	1.934 mL
10 mM	9.668 µL	48.34 µL	96.68 µL	483.4 µL	966.8 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1034.34 g/mol	=	mL	x	mM	=	mmol

References

View all 13 references: Citation Explorer

RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.

Authors:

Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S

Journal:

Methods in molecular biology (Clifton, N.J.) (2020): 27-35

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.

Authors:

Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M

Journal:

Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

Aligned Expression of IFI16 and STING Genes in RRMS Patients' Blood.

Authors:

Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid

Journal:

Endocrine, metabolic & immune disorders drug targets (2020): 878-886

Selenoprotein P inhibits cell proliferation and ROX production in HCC cells.

Authors:

Wang, Jianxin and Shen, Pei and Liao, Sha and Duan, Lian and Zhu, Dandan and Chen, Jinling and Chen, Liuting and Sun, Xiaolei and Duan, Yinong

Journal:

PloS one (2020): e0236491

Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA.

Authors:

Farzan, Valentina M and Kvach, Maksim V and Aparin, Ilya O and Kireev, Dmitry E and Prikazchikova, Tatiana A and Ustinov, Alexey V and Shmanai, Vadim V and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S

Journal:

Talanta (2019): 226-232

Physical properties

Molecular weight	1034.34
Solvent	DMSO

Spectral properties

Excitation (nm)	578
Emission (nm)	595

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on August 6, 2026