6-ROXtra™ acid

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1034.34
Solvent	DMSO

Spectral properties

Excitation (nm)	578
Emission (nm)	595

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1034.34

Excitation (nm)

578

Emission (nm)

595

Although ROX dyes have strong fluorescence they are notoriously unstable. Some commercial samples only have a few months’ shelf life. Compared to 6-ROX labeling compounds our 6-ROXtra™ dyes have greatly enhanced stability while they have essentially identical spectral properties to those of 6-ROX when conjugated to a biological substrate (such as oligonucleotides). In addition, our 6-ROXtra™ dyes are much more water-soluble than 6-ROX dyes that are essentially insoluble in water and aqueous buffers. Our preliminary studies indicated that 6-ROXtra-derived oligos are more easily purified than the corresponding 6-ROX oligos in most cases. When conjugated to proteins, the fluorescence of 6-ROX is severely quenched while 6-ROXtra™ still shows strong fluorescence.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROXtra™ acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.68 µL	483.4 µL	966.8 µL	4.834 mL	9.668 mL
5 mM	19.336 µL	96.68 µL	193.36 µL	966.8 µL	1.934 mL
10 mM	9.668 µL	48.34 µL	96.68 µL	483.4 µL	966.8 µL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	578
Emission (nm)	595

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-ROXtra™ SE Superior 6-ROX Replacement	578	595	-	-
6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer]	498	526	76000	0.12
6-JOE acid	520	545	75000¹	-
6-HEX acid	533	559	73000	0.15
6-TET Acid	521	542	73000	0.13

References

View all 13 references: Citation Explorer

Selenoprotein P inhibits cell proliferation and ROX production in HCC cells.
Authors: Wang, Jianxin and Shen, Pei and Liao, Sha and Duan, Lian and Zhu, Dandan and Chen, Jinling and Chen, Liuting and Sun, Xiaolei and Duan, Yinong
Journal: PloS one (2020): e0236491

Aligned Expression of IFI16 and STING Genes in RRMS Patients' Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.
Authors: Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 27-35

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA.
Authors: Farzan, Valentina M and Kvach, Maksim V and Aparin, Ilya O and Kireev, Dmitry E and Prikazchikova, Tatiana A and Ustinov, Alexey V and Shmanai, Vadim V and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Talanta (2019): 226-232

Expression of Plasma hsa-miR122 in HBV-Related Hepatocellular Carcinoma (HCC) in Vietnamese Patients.
Authors: Quoc, Nguyen Bao and Phuong, Nguyen Doan Nguyen and Ngan, Tang Kim and Linh, Nguyen Thi Minh and Cuong, Pham Hung and Chau, Nguyen Ngoc Bao
Journal: MicroRNA (Shariqah, United Arab Emirates) (2018): 92-99

Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82

[Short-term Effect of Roxithromycin on Abundance and Diversity of Ammonia-Oxidizing Microorganisms in Activated Sludge].
Authors: Gao, Jing-Feng and Sun, Li-Xin and Fan, Xiao-Yan and Pan, Kai-Ling and Li, Ding-Chang
Journal: Huan jing ke xue= Huanjing kexue (2017): 2961-2971

Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance.
Authors: Jung, Harin and Chung, Pil Joong and Park, Su-Hyun and Redillas, Mark Christian Felipe Reveche and Kim, Youn Shic and Suh, Joo-Won and Kim, Ju-Kon
Journal: Plant biotechnology journal (2017): 1295-1308