AAT Bioquest

6-ROXtra™ acid

Ordering information
Catalog Number
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1034.34
Spectral properties
Excitation (nm)578
Emission (nm)595
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Although ROX dyes have strong fluorescence they are notoriously unstable. Some commercial samples only have a few months’ shelf life. Compared to 6-ROX labeling compounds our 6-ROXtra™ dyes have greatly enhanced stability while they have essentially identical spectral properties to those of 6-ROX when conjugated to a biological substrate (such as oligonucleotides). In addition, our 6-ROXtra™ dyes are much more water-soluble than 6-ROX dyes that are essentially insoluble in water and aqueous buffers. Our preliminary studies indicated that 6-ROXtra-derived oligos are more easily purified than the corresponding 6-ROX oligos in most cases. When conjugated to proteins, the fluorescence of 6-ROX is severely quenched while 6-ROXtra™ still shows strong fluorescence.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROXtra™ acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM96.68 µL483.4 µL966.8 µL4.834 mL9.668 mL
5 mM19.336 µL96.68 µL193.36 µL966.8 µL1.934 mL
10 mM9.668 µL48.34 µL96.68 µL483.4 µL966.8 µL

Molarity calculator

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Spectral properties

Excitation (nm)578
Emission (nm)595

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-ROXtra™ SE *Superior 6-ROX Replacement*578595--
6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer]498526760000.12
6-JOE acid520545750001-
6-HEX acid533559730000.15
6-TET Acid521542730000.13


View all 13 references: Citation Explorer
Selenoprotein P inhibits cell proliferation and ROX production in HCC cells.
Authors: Wang, Jianxin and Shen, Pei and Liao, Sha and Duan, Lian and Zhu, Dandan and Chen, Jinling and Chen, Liuting and Sun, Xiaolei and Duan, Yinong
Journal: PloS one (2020): e0236491
Aligned Expression of IFI16 and STING Genes in RRMS Patients' Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886
SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30
RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.
Authors: Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 27-35
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA.
Authors: Farzan, Valentina M and Kvach, Maksim V and Aparin, Ilya O and Kireev, Dmitry E and Prikazchikova, Tatiana A and Ustinov, Alexey V and Shmanai, Vadim V and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Talanta (2019): 226-232
Expression of Plasma hsa-miR122 in HBV-Related Hepatocellular Carcinoma (HCC) in Vietnamese Patients.
Authors: Quoc, Nguyen Bao and Phuong, Nguyen Doan Nguyen and Ngan, Tang Kim and Linh, Nguyen Thi Minh and Cuong, Pham Hung and Chau, Nguyen Ngoc Bao
Journal: MicroRNA (Shariqah, United Arab Emirates) (2018): 92-99
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
[Short-term Effect of Roxithromycin on Abundance and Diversity of Ammonia-Oxidizing Microorganisms in Activated Sludge].
Authors: Gao, Jing-Feng and Sun, Li-Xin and Fan, Xiao-Yan and Pan, Kai-Ling and Li, Ding-Chang
Journal: Huan jing ke xue= Huanjing kexue (2017): 2961-2971
Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance.
Authors: Jung, Harin and Chung, Pil Joong and Park, Su-Hyun and Redillas, Mark Christian Felipe Reveche and Kim, Youn Shic and Suh, Joo-Won and Kim, Ju-Kon
Journal: Plant biotechnology journal (2017): 1295-1308