Name: 6-ROXtra™ SE *Superior 6-ROX Replacement*
Brand: AAT Bioquest
SKU: 397
Availability: InStock

6-ROXtra™ SE

Superior 6-ROX Replacement

Although ROX dyes have strong fluorescence they are notoriously unstable. Some commercial samples only have a few months' shelf life. Compared to 6-ROX labeling compounds our 6-ROXtra™ dyes have greatly enhanced stability while they have essentially identical spectral properties to those of 6-ROX when conjugated to a biological substrate (such as oligonucleotides). In addition, our 6-ROXtra™ dyes are much more water-soluble than 6-ROX dyes that are essentially insoluble in water and aqueous buffers. Our preliminary studies indicated that 6-ROXtra-derived oligos are more easily purified than the corresponding 6-ROX oligos in most cases. When conjugated to proteins, the fluorescence of 6-ROX is severely quenched while 6-ROXtra™ still shows strong fluorescence.

Stability comparison of 6-ROX versus 6-ROXtra™. Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 °C

Protocol

COA

Catalog	Size	Price	Quantity
397	5 mg	Price
401	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROXtra™ SE *Superior 6-ROX Replacement* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	97.066 µL	485.329 µL	970.657 µL	4.853 mL	9.707 mL
5 mM	19.413 µL	97.066 µL	194.131 µL	970.657 µL	1.941 mL
10 mM	9.707 µL	48.533 µL	97.066 µL	485.329 µL	970.657 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1030.23 g/mol	=	mL	x	mM	=	mmol

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis

Authors:

Faulds K, Smith WE, Graham D.

Journal:

Anal Chem (2004): 412

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry

Authors:

Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2004): 5488

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence

Authors:

Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.

Journal:

Langmuir (2004): 6834

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport

Authors:

Yang JH, Basinger SF, Gross RL, Wu SM.

Journal:

Invest Ophthalmol Vis Sci (2003): 1312

Physical properties

Molecular weight	1030.23
Solvent	DMSO

Spectral properties

Excitation (nm)	578
Emission (nm)	595

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 397)
Safety Data Sheet (Catalog 401)
Certificate of Analysis

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Page updated on October 25, 2025