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6-ROXtra™ SE *Superior 6-ROX Replacement*

Although ROX dyes have strong fluorescence they are notoriously unstable. Some commercial samples only have a few months' shelf life. Compared to 6-ROX labeling compounds our 6-ROXtra™ dyes have greatly enhanced stability while they have essentially identical spectral properties to those of 6-ROX when conjugated to a biological substrate (such as oligonucleotides). In addition, our 6-ROXtra™ dyes are much more water-soluble than 6-ROX dyes that are essentially insoluble in water and aqueous buffers. Our preliminary studies indicated that 6-ROXtra-derived oligos are more easily purified than the corresponding 6-ROX oligos in most cases. When conjugated to proteins, the fluorescence of 6-ROX is severely quenched while 6-ROXtra™ still shows strong fluorescence.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROXtra™ SE *Superior 6-ROX Replacement* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM97.066 µL485.329 µL970.657 µL4.853 mL9.707 mL
5 mM19.413 µL97.066 µL194.131 µL970.657 µL1.941 mL
10 mM9.707 µL48.533 µL97.066 µL485.329 µL970.657 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

References

View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Page updated on December 11, 2024

Ordering information

Price
Unit size
5 mg
1 mg
Catalog Number
397401
Quantity
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Additional ordering information

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Physical properties

Molecular weight

1030.23

Solvent

DMSO

Spectral properties

Excitation (nm)

578

Emission (nm)

595

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Stability comparison of 6-ROX versus 6-ROXtra&trade;. Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 &deg;C
Stability comparison of 6-ROX versus 6-ROXtra&trade;. Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 &deg;C
Stability comparison of 6-ROX versus 6-ROXtra&trade;. Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 &deg;C