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6-VIC phosphoramidite

6-VIC phosphoramidite is an asymmetrical xanthene dye widely used to prepare fluorescence-labeled oligonucleotides and design qPCR probes, such as TaqMan, Molecular Beacon, and Scorpion. Its spectral characteristics are similar to HEX and JOE, emitting fluorescence in the green-yellow range. 6-VIC phosphoramidite can efficiently label oligonucleotides at their 5'-end using standard or automated synthesis protocols. Protecting groups on the 6-VIC moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia.

Spectrum

References

View all 50 references: Citation Explorer
Tn5 tagments and transposes oligos to single-stranded DNA for strand-specific RNA sequencing.
Authors: Zhang, Yanjun and Tang, Yin and Sun, Zhongxing and Jia, Junqi and Fang, Yuan and Wan, Xinyi and Fang, Dong
Journal: Genome research (2023): 412-426
CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing.
Authors: Carbonell-Sala, Silvia and Lagarde, Julien and Nishiyori, Hiromi and Palumbo, Emilio and Arnan, Carme and Takahashi, Hazuki and Carninci, Piero and Uszczynska-Ratajczak, Barbara and Guigó, Roderic
Journal: bioRxiv : the preprint server for biology (2023)
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing.
Authors: Ge, Yang and van Roon, Lieke and Chen, H Sophia and Methorst, Ruben and Paton, Martin and DeRuiter, Marco C and Kielbasa, Szymon M and Jongbloed, Monique R M
Journal: Journal of visualized experiments : JoVE (2022)
Multiplexed Single-Nucleus RNA Sequencing Using Lipid-Oligo Barcodes.
Authors: Zhang, Qi and Kim, Seong Won and Gorham, Joshua M and DeLaughter, Daniel M and Ward, Tarsha and Seidman, Christine E and Seidman, Jonathan G
Journal: Current protocols (2022): e579
High-Throughput Activity Profiling of RNA-Cleaving DNA Catalysts by Deoxyribozyme Sequencing (DZ-seq).
Authors: Sednev, Maksim V and Liaqat, Anam and Höbartner, Claudia
Journal: Journal of the American Chemical Society (2022): 2090-2094
Page updated on October 11, 2024

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100 umoles
1 g
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Physical properties

Molecular weight

1023.38

Solvent

MeCN

Spectral properties

Excitation (nm)

526

Emission (nm)

543

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

1414265-81-8
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