AAT Bioquest

6-VIC phosphoramidite

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Physical properties
Molecular weight1023.38
Spectral properties
Excitation (nm)526
Emission (nm)543
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
6-VIC phosphoramidite is an asymmetrical xanthene dye widely used to prepare fluorescence-labeled oligonucleotides and design qPCR probes, such as TaqMan, Molecular Beacon, and Scorpion. Its spectral characteristics are similar to HEX and JOE, emitting fluorescence in the green-yellow range. 6-VIC phosphoramidite can efficiently label oligonucleotides at their 5'-end using standard or automated synthesis protocols. Protecting groups on the 6-VIC moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia.


Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of 6-VIC phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM97.715 µL488.577 µL977.154 µL4.886 mL9.772 mL
5 mM19.543 µL97.715 µL195.431 µL977.154 µL1.954 mL
10 mM9.772 µL48.858 µL97.715 µL488.577 µL977.154 µL

Molarity calculator

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Spectral properties

Excitation (nm)526
Emission (nm)543



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