6-VIC phosphoramidite

6-VIC phosphoramidite is an asymmetrical xanthene dye widely used to prepare fluorescence-labeled oligonucleotides and design qPCR probes, such as TaqMan, Molecular Beacon, and Scorpion. Its spectral characteristics are similar to HEX and JOE, emitting fluorescence in the green-yellow range. 6-VIC phosphoramidite can efficiently label oligonucleotides at their 5'-end using standard or automated synthesis protocols. Protecting groups on the 6-VIC moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia.

Protocol

SDS

COA

Catalog	Size	Price
6086	50 umoles	Price
6087	100 umoles	Price
6088	1 g	Price

References

View all 50 references: Citation Explorer

Tn5 tagments and transposes oligos to single-stranded DNA for strand-specific RNA sequencing.

Authors:

Zhang, Yanjun and Tang, Yin and Sun, Zhongxing and Jia, Junqi and Fang, Yuan and Wan, Xinyi and Fang, Dong

Journal:

Genome research (2023): 412-426

CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing.

Authors:

Carbonell-Sala, Silvia and Lagarde, Julien and Nishiyori, Hiromi and Palumbo, Emilio and Arnan, Carme and Takahashi, Hazuki and Carninci, Piero and Uszczynska-Ratajczak, Barbara and Guigó, Roderic

Journal:

bioRxiv : the preprint server for biology (2023)

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing.

Authors:

Ge, Yang and van Roon, Lieke and Chen, H Sophia and Methorst, Ruben and Paton, Martin and DeRuiter, Marco C and Kielbasa, Szymon M and Jongbloed, Monique R M

Journal:

Journal of visualized experiments : JoVE (2022)

Multiplexed Single-Nucleus RNA Sequencing Using Lipid-Oligo Barcodes.

Authors:

Zhang, Qi and Kim, Seong Won and Gorham, Joshua M and DeLaughter, Daniel M and Ward, Tarsha and Seidman, Christine E and Seidman, Jonathan G

Journal:

Current protocols (2022): e579

High-Throughput Activity Profiling of RNA-Cleaving DNA Catalysts by Deoxyribozyme Sequencing (DZ-seq).

Authors:

Sednev, Maksim V and Liaqat, Anam and Höbartner, Claudia

Journal:

Journal of the American Chemical Society (2022): 2090-2094

Physical properties

Molecular weight	1023.38
Solvent	MeCN

Spectral properties

Excitation (nm)	526
Emission (nm)	543

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	1414265-81-8

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 18, 2025