6-Fluorescein phosphoramidite

There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5'-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5'-hexachlorofluorescein-CE phosphoramidite (HEX) and 5'-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5'-end. Labeling the 3'-end of an oligo with fluorescein can be achieved using 3'-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.

Protocol

COA

Catalog	Size	Price	Quantity
6018	100 umoles	Price
6019	10x100 umoles	Price

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of 6-Fluorescein phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	85.009 µL	425.044 µL	850.087 µL	4.25 mL	8.501 mL
5 mM	17.002 µL	85.009 µL	170.017 µL	850.087 µL	1.7 mL
10 mM	8.501 µL	42.504 µL	85.009 µL	425.044 µL	850.087 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1176.35 g/mol	=	mL	x	mM	=	mmol

References

View all 13 references: Citation Explorer

Sequence-specific, self-reporting hairpin inversion probes

Authors:

Browne KA., undefined

Journal:

J Am Chem Soc (2005): 1989

Modified oligonucleotides containing lithocholic acid in their backbones: their enhanced cellular uptake and their mimicking of hairpin structures

Authors:

Kim SJ, Bang EK, Kwon HJ, Shim JS, Kim BH.

Journal:

Chembiochem (2004): 1517

Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis

Authors:

Dobson N, McDowell DG, French DJ, Brown LJ, Mellor JM, Brown T.

Journal:

Chem Commun (Camb) (2003): 1234

Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases

Authors:

Kutyavin IV, Lokhov SG, Afonina IA, Dempcy R, Gall AA, Gorn VV, Lukhtanov E, Metcalf M, Mills A, Reed MW, S and ers S, Shishkina I, Vermeulen NM.

Journal:

Nucleic Acids Res (2002): 4952

Diels--Alder bioconjugation of diene-modified oligonucleotides

Authors:

Hill KW, Taunton-Rigby J, Carter JD, Kropp E, Vagle K, Pieken W, McGee DP, Husar GM, Leuck M, Anziano DJ, Sebesta DP.

Journal:

J Org Chem (2001): 5352

Physical properties

Molecular weight	1176.35
Solvent	MeCN

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	204697-37-0

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025