Name: Amplite® Colorimetric Acetylcholinesterase Assay Kit
Brand: AAT Bioquest
SKU: 11400
Price: 232 USD
Availability: InStock

Amplite® Colorimetric Acetylcholinesterase Assay Kit

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity- each molecule of AChE degrades about 5000 molecules of acetylcholine per second. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface. This Amplite® Colorimetric Acetylcholinesterase Assay Kit provides a convenient method for the detecting AChE activity. The kit uses DTNB to quantify the thiolcholine produced from the hydrolysis of acetylthiolcholine by AChE. The absoprtion intensity of DTNB adduct is proportional to the formation of thiolcholine, thus the AChE activity.

Acetylcholinesterase dose response was measured in a white/clear bottom 96-well plate with Amplite® Colorimetric Acetylcholinesterase Assay Kit using a SpectraMax microplate reader (Molecular devices). As low as 0.1 mU/well of Acetylcholinesterase can be detected with 30 minutes incubation(n=3).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11400	200 Tests	Price

Citations

View all 64 citations: Citation Explorer

A Hierarchical Short Microneedle-Cupping Dual-Amplified Patch Enables Accelerated, Uniform, Pain-Free Transdermal Delivery of Extracellular Vesicles

Authors:

Song, Minwoo and Ha, Minji and Shin, Sol and Kim, Minjin and Son, Soyoung and Lee, Jihyun and Hwang, Gui Won and Kim, Jeongyun and Duong, Van Hieu and Park, Jae Hyung and others,

Journal:

Nano-Micro Letters (2026): 1--22

Acceleration-Dependent Effects of Vibrotactile Gamma Stimulation on Cognitive Recovery and Cholinergic Function in a Scopolamine-Induced Neurotoxicity Mouse Model

Authors:

Kim, Tae-Woo and Park, Hee-Jung and Nam, Myeong-Hyun and Lee, In-Ho and Chen, Zu-Yu and Yun, Hee-Deok and Seo, Young-Kwon

Journal:

Biomedicines (2025): 2031

The complex interplay between ligand strain, coulombic interactions, and binding site dynamics of two congeneric AChE inhibitors

Authors:

Babu, Aravinda and Remya, Chandran and Sajith, AM and Dileep, KV and Kenchaiah, Sunil and Khade, Ajit and Zyryanov, Grigory V and Joy, Muthipeedika Nibin

Journal:

Journal of Molecular Structure (2025): 141479

Identifying Organic Chemicals with Acetylcholinesterase Inhibition in Nationwide Estuarine Waters by Machine Learning-Assisted Mass Spectrometric Screening

Authors:

Wang, Haotian and Feng, Xiaoxia and Su, Wenyuan and Zhong, Laijin and Liu, Yanna and Liang, Yong and Ruan, Ting and Jiang, Guibin

Journal:

Environmental Science \& Technology (2024)

Isolation of new indole alkaloid triglucoside from the aqueous extract of Uncaria rhynchophylla

Authors:

Koseki, Yuta and Nishimura, Hiroaki and Asano, Ryuji and Aoki, Katsuyuki and Shiyu, Li and Sugiyama, Ryosuke and Yamazaki, Mami

Journal:

Journal of Natural Medicines (2024): 1--8

References

View all 41 references: Citation Explorer

Expression and DNA microarray analysis of a platelet activating factor-related molecule in severe pneumonia in mice due to influenza virus and bacterial co-infection

Authors:

Seki M, Kosai K, Hara A, Imamura Y, Nakamura S, Kurihara S, Izumikawa K, Kakeya H, Yamamoto Y, Yanagihara K, Miyazaki Y, Mukae H, Tashiro T, Kohno S.

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Jpn J Infect Dis (2009): 6

Protective effect of stilbenes containing extract-fraction from Cajanus cajan L. on Abeta(25-35)-induced cognitive deficits in mice

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Ruan CJ, Si JY, Zhang L, Chen DH, Du GH, Su L.

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Neurosci Lett (2009): 159

Platelet activating factor (PAF) and activity of its biosynthetic and catabolic enzymes in blood and leukocytes of male patients with newly diagnosed heart failure

Authors:

Detopoulou P, Nomikos T, Fragopoulou E, Antonopoulou S, Kotroyiannis I, Vassiliadou C, Panagiotakos DB, Chrysohoou C, Pitsavos C, Stefanadis C.

Journal:

Clin Biochem (2009): 44

Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via phospholipase C/diacylglycerol and phospholipase A2/omega-hydroxylase/20-HETE pathways

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Inoue R, Jensen LJ, Jian Z, Shi J, Hai L, Lurie AI, Henriksen FH, Salomonsson M, Morita H, Kawarabayashi Y, Mori M, Mori Y, Ito Y.

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Neuroprotective role of Convolvulus pluricaulis on aluminium induced neurotoxicity in rat brain

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Bihaqi SW, Sharma M, Singh AP, Tiwari M.

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J Ethnopharmacol (2009): 409

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Absorbance microplate reader
Absorbance	410 ± 5 nm
Recommended plate	Clear bottom

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Page updated on September 25, 2025

Component A: DTNB	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: Acetylthiocholine	1 vial
Component D: Acetylcholinesterase Standard	1 vial (5 units)