Name: Amplite® Colorimetric NADH Assay Kit
Brand: AAT Bioquest
SKU: 15271
Price: 296 USD
Availability: InStock

Amplite® Colorimetric NADH Assay Kit

Amplite® Colorimetric NADH Assay Kit is a colorimetric assay kit for quantifying NADH using an enzyme-free chromogenic probe in a microplate format.

Colorimetric detection: Uses an NADH-responsive chromogenic probe with absorbance at 460 nm.
High sensitivity: Detects as little as 3 µM NADH in a 100 µL assay volume.
Enzyme-free chemistry: Directly recognizes NADH without an enzyme-coupled reaction.
Microplate compatible: Supports 96-well or 384-well formats.
Applications: Suitable for NADH quantitation in metabolic and redox research samples.

NADH dose response was measured with Amplite® Colorimetric NADH Assay Kit in a 96-well white/clear bottom plate using a SpectraMax microplate reader (Molecular devices).

Protocol

SDS

Catalog	Size	Price	Quantity
15271	400 Tests	Price

Overview

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+. NAD forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. The traditional NAD/NADH and NADP/NADPH assays are based on monitoring the changes in NADH or NADPH absorption at 340 nm. The short UV wavelength of NAD/NADH and NADP/NADPH assays makes the traditional methods suffer low sensitivity and high interference. Due to the weak absorption of NAD and NADH, the UV absorption method requires large sample sizes, making NAD and NADH measurements unpractical for limited sample size. AAT Bioquest's Amplite® Colorimetric NADH Assay Kit provides a convenient method for the detection of NADH. The NADH probe is a chromogenic sensor that has its maximum absorbance at 460 nm upon NADH reduction. The absorbance increase at 460 nm is directly proportional to the concentration of NADH in the solution. The NADH probe can recognize NADH in an enzyme-free reaction, and the signal can be easily read by an absorbance microplate reader at ~460 nm. The Amplite® Colorimetric NADH Assay Kit provides a sensitive assay to detect as little as 3 µM NADH in a 100 µL assay volume. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

Citations

View all 59 citations: Citation Explorer

Protein lysine acetylation does not contribute to the high rates of fatty acid oxidation seen in the post-ischemic heart

Authors:

Ketema, Ezra B and Ahsan, Muhammad and Zhang, Liyan and Karwi, Qutuba G and Lopaschuk, Gary D

Journal:

Scientific Reports (2024): 1193

The Linkage of Yeast Metabolites, Produced Under Hyperosmotic Stress, to Cellular Cofactor Systems During Icewine Fermentation.

Authors:

Allie, Robert

Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+/NADH ratio

Authors:

Luo, Gang and Huang, Bingqing and Qiu, Xiang and Xiao, Lin and Wang, Ning and Gao, Qin and Yang, Wei and Hao, Liping

Journal:

Molecular Nutrition & Food Research (2017)

Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase

Authors:

Ling, Min and Huang, Peixin and Islam, Shamima and Heruth, Daniel P and Li, Xuanan and Zhang, Li Qin and Li, Ding-You and Hu, Zhaohui and Ye, Shui Qing

Journal:

Cell & Bioscience (2017): 27

MCU-dependent mitochondrial Ca2+ inhibits NAD+/SIRT3/SOD2 pathway to promote ROS production and metastasis of HCC cells

Authors:

Ren, T and Zhang, H and Wang, J and Zhu, J and Jin, M and Wu, Y and Guo, X and Ji, L and Huang, Q and Yang, H and others, undefined

Journal:

Oncogene (2017)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	460 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: NADH/NADPH Probe	1 bottle (4 mL)
Component B: Assay Buffer	1 bottle (16 mL)
Component C: NADH Standard (FW: 709)	1 vial (142 µg, lyophilized )
Component D: Lysis Buffer	1 bottle (4 mL)