Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Acetylcholinesterase Assay Kit

Red Fluorescence

Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantification of acetylcholinesterase activity in a microplate format.

Detection Principle: Quantifies choline generated from acetylcholine hydrolysis.
Sensitivity: Detects AChE activity down to 0.1 mU/mL.
Chemistry: Uses Amplite® Red with choline oxidase coupling.
Instrument Compatibility: Fluorescence detection at Ex 540/Em 590 nm.

Acetylcholinesterase dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Acetylcholinesterase Assay Kit using a Gemini fluorescence microplate reader (Molecular devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11402	200 Tests	Price

Overview

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity- each molecule of AChE degrades about 5000 molecules of acetylcholine per second. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface. This Amplite® Fluorimetric Acetylcholinesterase Assay Kit provides one of the most sensitive methods for the detecting AChE activity. The kit uses Amplite® Red to quantify the choline produced from the hydrolysis of acetylcholine by AChE through choline oxidase-mediated enzyme coupling reactions. The fluorescence intensity of Amplite® Red™ is proportional to the formation of choline, thus the AChE activity.

Citations

View all 35 citations: Citation Explorer

ME as a Multi-Target-Directed Ligand for Alzheimer’s Disease: A Promising Lead for Drug Design

Authors:

Aiswarya, N and Remya, Chandran and Sajitha, K and Rajan, Rakhi and Vasudevan, DM and Dileep, KV

Journal:

ASPET Discovery (2025): 100019

The complex interplay between ligand strain, coulombic interactions, and binding site dynamics of two congeneric AChE inhibitors

Authors:

Babu, Aravinda and Remya, Chandran and Sajith, AM and Dileep, KV and Kenchaiah, Sunil and Khade, Ajit and Zyryanov, Grigory V and Joy, Muthipeedika Nibin

Journal:

Journal of Molecular Structure (2025): 141479

Aluminum chloride and D-galactose induced a zebrafish model of Alzheimer's disease with cognitive deficits and aging

Authors:

Luo, Li and Yan, Tao and Yang, Le and Zhao, Minggao

Journal:

Computational and Structural Biotechnology Journal (2024)

Identification of Potent and Selective Acetylcholinesterase/Butyrylcholinesterase Inhibitors by Virtual Screening

Authors:

Xu, Tuan and Li, Shuaizhang and Li, Andrew J and Zhao, Jinghua and Sakamuru, Srilatha and Huang, Wenwei and Xia, Menghang and Huang, Ruili

Journal:

Journal of Chemical Information and Modeling (2023)

Hepatic vagus nerve regulates Kupffer cell activation via α7 nicotinic acetylcholine receptor in nonalcoholic steatohepatitis

Authors:

Nishio, Takahiro and Taura, Kojiro and Iwaisako, Keiko and Koyama, Yukinori and Tanabe, Kazutaka and Yamamoto, Gen and Okuda, Yukihiro and Ikeno, Yoshinobu and Yoshino, Kenji and Kasai, Yosuke and others, undefined

Journal:

Journal of Gastroenterology (2017): 1--12

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 6, 2026

Component A: Amplite® Red	1 vial
Component B: Acetylcholinesterase Probe	2 bottles (lyophilized powder)
Component C: Acetylcholine	1 vial
Component D: Acetylcholinesterase Standard	1 vial (5 units)
Component E: Assay Buffer	1 bottle (10 mL)
Component F: Dilution Buffer	1 bottle (10 mL)
Component G: DMSO	1 vial (100 µL)