logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Amplite® Fluorimetric Acetylcholinesterase Assay Kit
Red Fluorescence
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity- each molecule of AChE degrades about 5000 molecules of acetylcholine per second. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface. This Amplite® Fluorimetric Acetylcholinesterase Assay Kit provides one of the most sensitive methods for the detecting AChE activity. The kit uses Amplite® Red to quantify the choline produced from the hydrolysis of acetylcholine by AChE through choline oxidase-mediated enzyme coupling reactions. The fluorescence intensity of Amplite® Red™ is proportional to the formation of choline, thus the AChE activity.
Acetylcholinesterase dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Acetylcholinesterase Assay Kit using a Gemini fluorescence microplate reader (Molecular devices).
Acetylcholinesterase dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Acetylcholinesterase Assay Kit using a Gemini fluorescence microplate reader (Molecular devices).
CatalogSize
Price
Quantity
11402200 Tests
Price
 
Spectral properties

Excitation (nm)571
Emission (nm)584
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
Contact us

Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on September 25, 2025