Name: Amplite® Rapid Colorimetric Biotin Quantitation Kit *Optimized to Use with Nanodrop*
Brand: AAT Bioquest
SKU: 5537
Price: 237 USD
Availability: InStock

Amplite® Rapid Colorimetric Biotin Quantitation Kit

Optimized to Use with Nanodrop

Amplite® Rapid Colorimetric Biotin Quantitation Kit is a colorimetric assay kit for quantification of biotin attached to proteins or other macromolecules using a NanoDrop™ spectrophotometer.

Detection Principle: Biotin displaces HABA from the HABA/avidin complex, decreasing absorbance at 500 nm.
Quantitation Range: Measures biotin concentrations from 2–16 µM.
Workflow: Requires only 2–2.5 µL of sample for NanoDrop™ measurement after a 5-minute incubation.
Instrument Compatibility: Optimized for NanoDrop™ spectrophotometric detection at 500 nm.

General packaging for Cat. No. 5537. Your actual packaging may differ.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5537	100 tests	Price

Overview

Amplite® Colorimetric Biotin Quantitation Kit is designed to quantify the number of biotins attached to a protein or other macromolecule with a Nanodrop Spectrometer. The kit uses HABA (4’-hydroxyazobenzene-2-carboxylic acid) reagent that demonstrates a dramatic spectral change when bound to avidin. Biotin easily displaces HABA from the HABA/avidin complex, resulting in a decrease of absorption at 500 nm. By measuring the absorbance change before and after the addition of the biotin-containing sample at 500 nm, the amount of biotin in a sample can be quantified spectrophotometrically. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. It is optimized to use with Nanodrop spectrometer. Biotin is a relatively small molecule that is routinely conjugated to antibodies and proteins with minimal interference of their biological activity. The avidin/streptavidin-biotin interaction is the strongest known binding pair between a protein and its ligand. The biotin-avidin interaction has been extensively explored for a variety of biological applications.

References

View all 50 references: Citation Explorer

Quantification of Hepatitis E Virus ORF2 Protein by a Novel Sandwich ELISA.

Authors:

Subramaniam, Sakthivel and Fares-Gusmao, Rafaelle and McGivern, David R

Journal:

Viruses (2024)

A picogram BA-ELISA quantification assay for rLj-RGD3, a platelet fibrinogen receptor antagonist, in the rat plasma and its application to a pharmacokinetic study.

Authors:

Wang, Yuping and Liu, Zhien and Han, Guozhu and Yu, Ping and Yang, Xiaobo and Wang, Jihong and Lv, Li

Journal:

PLoS neglected tropical diseases (2023): e0011568

Simultaneous quantification of multiple single nucleotide variants in PIK3CA ctDNA using mass-tagged LCR probe sets.

Authors:

Wang, Zhongcheng and Li, Li and Kuang, Yuqiong and Yao, Jiang and Xu, Feifei and Chen, Yun

Journal:

Talanta (2023): 124426

Quantification of 10 B vitamins in mouse colon by LC-MS/MS: Application on breast cancer mice treated with doxorubicin.

Authors:

Wu, Jing and Xu, Yan and Yang, Jiahong and Yu, Xinyue and Han, Zhaodi and Guo, Linling and Huang, Yin and Zhang, Yuxin

Journal:

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2023): 123714

Mitochondrial Replication Assay (MIRA) for Efficient in situ Quantification of Nascent mtDNA and Protein Interactions with Nascent mtDNA (mitoSIRF).

Authors:

Lozen, Macy and Chen, Yue and Boisvert, Rebecca A and Schlacher, Katharina

Journal:

Bio-protocol (2023): e4680

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 24, 2026

Component A: Avidin	1 Vial
Component B: HABA Assay Buffer	2 Vials (1mL/Vial)
Component C: d-Biotin	1 Vial (200 µL, 100 µM)