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Amplite® Universal Fluorimetric MMP Activity Assay Kit
Green Fluorescence
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair of tissue damage. While the exact contribution of MMPs to certain pathological processes is difficult to assess, MMPs appear to play a key role in the development of arthritis as well as in the invasion and metastasis of cancer. MMPs tend to have multiple substrates, with most family members having the ability to degrade different types of collagen along with elastin, gelatin and fibronectin. It is quite difficult to find a substrate that is selective to a single MMP enzyme. This kit is designed to check the general activity of a MMP enzyme. It can also be used to screening MMP inhibitors when a purified MMP enzyme is used. We also offer a few MMP enzyme of high activity.
Detection of MMPs activity using Amplite® Universal Fluorimetric MMP Activity Assay Kit. The APMA-activated MMPs, 30 ng each, were mixed with MMP Green™ substrate. The fluorescence signal was monitored one hour after starting the the reaction by using a NOVOStar microplate reader (BMG Labtech) with a filter set of Ex/Em = 490/525 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Green™ substrate but no MMPs. The MMP Green™ substrate can detect the activity of sub-nanogram of all MMPs (n=3).
Detection of MMPs activity using Amplite® Universal Fluorimetric MMP Activity Assay Kit. The APMA-activated MMPs, 30 ng each, were mixed with MMP Green™ substrate. The fluorescence signal was monitored one hour after starting the the reaction by using a NOVOStar microplate reader (BMG Labtech) with a filter set of Ex/Em = 490/525 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Green™ substrate but no MMPs. The MMP Green™ substrate can detect the activity of sub-nanogram of all MMPs (n=3).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13510100 Tests
Price
 
Spectral properties
Excitation (nm)494
Emission (nm)515
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
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Page updated on September 25, 2025