Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

Amplite® Universal Fluorimetric MMP Activity Assay Kit

Green Fluorescence

Amplite® Universal Fluorimetric MMP Activity Assay Kit is a fluorimetric assay kit for detection of general matrix metalloproteinase activity in biological samples.

Detection Principle: Uses MMP Green™ substrate to generate increased green fluorescence upon MMP cleavage.
Sensitivity: Detects sub-nanogram amounts of MMPs under AAT’s demonstrated assay conditions.
Specificity: Measures general MMP activity rather than a single MMP subtype.
Instrument Compatibility: Supports 96- or 384-well formats with fluorescence detection at Ex/Em 490/525 nm.

Detection of MMPs activity using Amplite® Universal Fluorimetric MMP Activity Assay Kit. The APMA-activated MMPs, 30 ng each, were mixed with MMP Green™ substrate. The fluorescence signal was monitored one hour after starting the the reaction by using a NOVOStar microplate reader (BMG Labtech) with a filter set of Ex/Em = 490/525 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Green™ substrate but no MMPs. The MMP Green™ substrate can detect the activity of sub-nanogram of all MMPs (n=3).

Protocol

SDS

Catalog	Size	Price	Quantity
13510	100 Tests	Price

Overview

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair of tissue damage. While the exact contribution of MMPs to certain pathological processes is difficult to assess, MMPs appear to play a key role in the development of arthritis as well as in the invasion and metastasis of cancer. MMPs tend to have multiple substrates, with most family members having the ability to degrade different types of collagen along with elastin, gelatin and fibronectin. It is quite difficult to find a substrate that is selective to a single MMP enzyme. This kit is designed to check the general activity of a MMP enzyme. It can also be used to screening MMP inhibitors when a purified MMP enzyme is used. We also offer a few MMP enzyme of high activity.

Citations

View all 34 citations: Citation Explorer

Alleviation of Oxidative Stress Upregulates Protective Autophagy to Mediate PTX3-Induced Anoikis Resistance and Metastasis in HNSCC

Authors:

Chen, Ben-Kuen and Chang, Ting-Wei and Huang, Wan-Chen and Chan, Shih-Hung and Tsai, Jhih-Peng and Chiou, Jing-Ting and Chang, Hsiao-Yu and Meow, Ying-Zhu and Chen, Tsan-Yu and Wang, Ju-Ming and others,

MMP8 mediates CD115 cleavage-dependent elimination of large peritoneal macrophages in response to microbial exposure

Authors:

Giri, Shilpi and Martin, Andrew T and Safronova, Alexandra and Eliseeva, Sophia I and Ahmed, Sara and Kwok, Samantha F and Yarovinsky, Felix

Journal:

Cell Reports (2026): 117234

The Loss of Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) Alters Endothelial Cell Permeability, Metabolism, and Survival During Infectious Challenge

Authors:

Fontes, Milene Tavares and Lamb, Celia K and Pourquoi, Allison and Atzrodt, Cassandra Lauren and Nguyen, Hong-Ngan and Panja, Sourav and Stark, Ryan Jordan

Journal:

Clinical Science (2026): CS20256682

Mechanical Stimulation of Equine Bone Marrow Mesenchymal Stromal Cell-Derived Cartilage-Like In Vitro Model Triggers Osteoarthritis Features

Authors:

Contentin, Romain and Jehl, Cassie and Commenchail, Kevin and Legendre, Florence and Gal{\'e}ra, Philippe and Cass{\'e}, Fr{\'e}d{\'e}ric and Demoor, Magali

Journal:

ACS Biomaterials Science \& Engineering (2025)

A Smart Functional Microneedle, Tethered with Neuropeptide S and Tissue Inhibitor of Metalloproteinases, Promotes Skin, Vascular, and Nerve Regeneration for Chronic Diabetic Skin Wound Healing

Authors:

Lu, Xiaojie and Yan, Yan and Cheng, Mengdan and Wu, Yiqing and Zhang, Liangliang and Xu, Bai

Journal:

Small Structures (2025): 2500278

Spectral properties

Excitation (nm)	494
Emission (nm)	515

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 28, 2026

Component A: MMP Green™ Substrate	1 vial (60 µL)
Component B: APMA, 4-Aminophenylmercuric Acetate	1 vial (20 µL, 1 M)
Component C: Assay Buffer	1 bottle (20 mL)