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Anti-17-alpha-hydroxyprogesterone antibody *Mouse anti-human, monoclonal IgG1*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application CLIA |
17 alpha-hydroxyprogesterone (17 α-OHP) is a steroid hormone synthesized from progesterone. It acts as an agonist of the progesterone receptor and is a precursor in the biosynthesis of many other steroids such as glucocorticoids, androgens and estrogens. In clinical biology, measuring 17 α-OHP levels in the blood are useful in the detection and evaluation of congenital adrenal hyperplasia (CAH). Patients with CAH express insufficient amounts of the enzyme 21-hydroxylase, which is necessary for the conversion of 17 α-OHP into cortisol. As a result, 17 α-OHP levels in the blood build up. Anti-17 α-OHP monoclonal antibodies are highly sensitive for the detection of 17 α-OHP. They can be used for a broad range of immunoassays such as competitive chemiluminescence immunoassays (CLIA) and ELISA.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-17-alpha-hydroxyprogesterone antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
| AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
| Small Molecules | Biotin |
| Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
| iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
| mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Clinical comparison of AAT Bioquest’s 17 α -OHP immunoassay and a competitor’s immunoassay: 27 clinical blood samples were tested using 17 α -OHP monoclonal antibodies from AAT Bioquest on a CLIA platform. Data from this study were compared to that of a competitor’s diagnostic kit.
Figure 2. Competition curve for 17 α -OHP in competitive chemiluminescence immunoassay (CLIA): monoclonal antibodies and 17α-OHP standards were added to a microplate pre-coated with 17 α-OHP conjugates. Secondary detection antibodies were labeled with horseradish peroxidase (HRP). Quantity of HRP combination was inversely proportional to the amount of standard.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?