Anti-Alpha-1-microglobulin protein antibody *Mouse anti-human, monoclonal IgG2a*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application LETIA |
Alpha-1-microglobulin (A1M or protein HC) is a 26-kDa glycoprotein belonging to the lipocalin family. It is widely distributed in body fluids both in free form and complexed with immunoglobulin A (IgA). A1M acts as a radical scavenger. It protects cells and tissue against oxidative damage by binding and degrading heme as well as reductase. High concentrations of A1M in urine or serum may indicate acute kidney injury, proteinuria and nephropathy in type-2 diabetic subjects. Anti-A1M monoclonal antibodies are highly sensitive for the detection of AMBP proteins. They can be used for a broad range of immunoassays such as latex enhanced turbidimetric immunoassays (LETIA).
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Alpha-1-microglobulin protein antibody *Mouse anti-human, monoclonal IgG2a* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
| AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
| Small Molecules | Biotin |
| Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
| iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
| mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Clinical comparison between AAT Bioquest’s A1M immunoassay and commercial diagnostic kit: 20 clinical blood samples were separately tested using A1M antibody in LETIA platform. Data from this study were compared to that of commercial diagnostic kit.
Figure 2. Calibration curve for A1M in latex-enhanced turbidimetric immunoassay (LETIA): Anti-A1M Clone 1 (Cat# V100035) and Anti-A1M Clone 2 (Cat#V100040) were pre-coated onto latex beads to form insoluble complexes, resulting in turbidity increasing. Absorbance increase was monitored by automatic biochemical analyzer. The calibration curve was fitted according to the relationship between absorbance values and A1M concentrations. (Detection sensitivity ranging from 0 mg/L to 160 mg/L)