Anti-C-reactive protein antibody *Mouse anti-human, monoclonal IgG*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application LETIA |
C-reactive protein (CRP) is an annular, pentameric protein synthesized by the liver. It is present in blood plasma and is used primarily as a biomarker of inflammation. In response to inflammation, infection, or tissue damage, the level of CRP in human serum can increase 1000-fold. CRP also exhibits Ca2+ dependent binding to ligands, promote phagocytosis, and can induce production of hydrogen peroxide and inflammatory cytokines by monocytes. In clinical biology, CRP concentration can be used to evaluate the onset of cardiovascular disease. Anti-CRP monoclonal antibodies are highly specific for the detection of human C-reactive proteins. They can be used for a broad range of immunoassays such as lateral-flow immunoassay (LFIA), chemiluminescent immunoassay (CLIA), and latex enhanced turbidimetric immunoassays (LETIA).
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-C-reactive protein antibody *Mouse anti-human, monoclonal IgG* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
| AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
| Small Molecules | Biotin |
| Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
| iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
| mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Determination of clinical samples using AAT Bioquest’s mAb on CLIA platform: a study was performed where 48 specimens were tested using two antibody combinations and compared to a high-quality comparison assay.
Figure 2. Calibration curve for CRP in latex-enhanced turbidimetric immunoassay (LETIA): anti-CRP monoclonal antibodies (Cat#V100150) pre-coated onto latex beads were reacted with CRP proteins to form insoluble complexes, resulting in turbidity increasing. Absorbance increase was monitored by automatic biochemical analyzer.
Figure 3. Semi-quantitative detection of CRP protein in colloidal gold immunochromatogragphic assay: a set of CRP calibrators with concentrations of 0, 0.1, 0.5, 2, 10, 20, 50 and 100 mg/L were detected using an LFIA platform. The capture antibody was stripped on the nitrocellulose membrane, and the detection antibody was conjugated to colloidal gold. The best selected mAb combination is (capture-detection): Cat# V100150 (Clone 6) – Cat# V100150 (Clone 5).
Figure 4. Calibration curve for CRP in sandwich chemiluminescence immunoassay (CLIA): two monoclonal antibodies were tested in pairs as capture and detection antibodies for the development of a quantitative sandwich immunoassay. The mAb combination for quantification of human CRP is (capture-detection): Cat# V100150 (Clone 3) – Cat# V100150 (Clone 2).
Figure 5. Clinical comparison of AAT Bioquest’s CRP immunoassay and commercial diagnostic assay.
Figure 6. Semi-quantitative detection of the concentration of the CRP in the blood samples (serum were directly added onto strips without dilution). 20 samples from healthy donors and patients with acute or chronic inflammation were detected using AAT Bioquest’s LFIA CRP assay.