Anti-C-reactive protein antibody Mouse anti-human, monoclonal IgG2b

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

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Intended use

Research Use Only (RUO)

Overview SDS Protocol

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-C-reactive protein antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

Images

Figure 1. Determination of clinical samples using AAT Bioquest’s mAb on CLIA platform: a study was performed where 48 specimens were tested using two antibody combinations and compared to a high-quality comparison assay.

Figure 2. Calibration curve for CRP in latex-enhanced turbidimetric immunoassay (LETIA): anti-CRP monoclonal antibodies (Cat#V100150) pre-coated onto latex beads were reacted with CRP proteins to form insoluble complexes, resulting in turbidity increasing. Absorbance increase was monitored by automatic biochemical analyzer.

Semi-quantitative detection of CRP protein in colloidal gold immunochromatogragphic assay: a set of CRP calibrators with concentrations of 0, 0.1, 0.5, 2, 10, 20, 50 and 100 mg/L were detected using an LFIA platform. The capture antibody was stripped on the nitrocellulose membrane, and the detection antibody was conjugated to colloidal gold. The best selected mAb combination is (capture-detection): Cat# V100150 (Clone 6) – Cat# V100150 (Clone 5).

Figure 3. Semi-quantitative detection of CRP protein in colloidal gold immunochromatogragphic assay: a set of CRP calibrators with concentrations of 0, 0.1, 0.5, 2, 10, 20, 50 and 100 mg/L were detected using an LFIA platform. The capture antibody was stripped on the nitrocellulose membrane, and the detection antibody was conjugated to colloidal gold. The best selected mAb combination is (capture-detection): Cat# V100150 (Clone 6) – Cat# V100150 (Clone 5).

Figure 4. Calibration curve for CRP in sandwich chemiluminescence immunoassay (CLIA): two monoclonal antibodies were tested in pairs as capture and detection antibodies for the development of a quantitative sandwich immunoassay. The mAb combination for quantification of human CRP is (capture-detection): Cat# V100150 (Clone 3) – Cat# V100150 (Clone 2).

Figure 5. Clinical comparison of AAT Bioquest’s CRP immunoassay and commercial diagnostic assay.

Semi-quantitative detection of the concentration of the CRP in the blood samples (serum were directly added onto strips without dilution). 20 samples from healthy donors and patients with acute or chronic inflammation were detected using AAT Bioquest’s LFIA CRP assay.

Figure 6. Semi-quantitative detection of the concentration of the CRP in the blood samples (serum were directly added onto strips without dilution). 20 samples from healthy donors and patients with acute or chronic inflammation were detected using AAT Bioquest’s LFIA CRP assay.