Anti-Cystatin-C protein antibody Mouse anti-human, monoclonal IgG1

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C)

Overview SDS Protocol

See also: Antibodies and Proteomics

Other names

Host

mouse

Reactivity

human

Application

LETIA

Cystatin C (CYSC) is a non-glycosylated, low molecular weight protein synthesized by most nucleated cells. It is present in all tissues and body fluids acting as a potent inhibitor of lysosomal proteinases and as an extracellular inhibitor of cysteine proteases. CYSC is primarily used a biomarker of renal function, but also has associations with obesity, insulin resistance, hypertension, and cardiovascular mortality. Under normal conditions, CYSC in serum is 0.47 " 1.09 mg/L. When renal function is impaired, CYCS concentration in blood will change with glomerular filtration rate (GFR). During renal failure, GFR decreases while CYSC blood concentration increases more than 10-fold. Anti-CYSC monoclonal antibodies are highly sensitive for the detection of human CYSC proteins. They can be used for a broad range of CYSC immunoassays such as latex enhanced turbidimetric immunoassays (LETIA).

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-Cystatin-C protein antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

Images

Figure 1. Clinical comparison of AAT Bioquest’s CYSC reagent and commercial kit.

Figure 2. Calibration curve for CYSC LETIA assay: human CYSC were reacted with anti-human CYSC antibody-coated latex, resulting in agglutination and increase in turbidity. Turbidity changes were monitored using a spectrometer to quantitatively measure the CYSC concentration in the sample.

Figure 3. Effect of repeated freeze-thaw on CYSC antibodies: anti-CYSC monoclonal antibodies (Cat# V100030) were subjected to subsequent freeze-thaw cycles from -20°C to 20°C. Anti-CYSC monoclonal antibodies were stable after five repeated freeze-thaw cycles.