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Anti-Epididymal secretory protein 4 antibody *Mouse anti-human, monoclonal IgG1*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application ELISA; CLIA |
Human epididymis secretory protein E4 (HE4) is a protein encoded by the WFDC2 gene in humans. This gene is expressed primarily in pulmonary epithelial cells, but has found to be expressed in some ovarian cancers. As such, HE4 serves as a serum biomarker in women with a pelvic mass to distinguish benign from malignant disease and as a tumor marker for ovarian cancer. Anti-HE4 monoclonal antibodies are highly sensitive for the detection of human HE4. They can be used for a broad range of immunoassays such as chemiluminescence immunoassay (CLIA) and rapid sandwich immunoassays.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Epididymal secretory protein 4 antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
| AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
| Small Molecules | Biotin |
| Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
| iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
| mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Determination of clinical samples using two MAb combinations on CLIA platform: 40 specimens were tested using two antibody combinations on a CLIA platform and a commercial comparison assay. The antibody combinations used were (capture-detection respectively): Mab combination A: Cat# V100060 Clone 2- Cat# V100060 Clone 3;Mab combination B: Cat# V100060 Clone 3- Cat# V100060 Clone 1.
Figure 2. Calibration curves for HE4 in sandwich chemiluminescence immunoassay (CLIA): all monoclonal antibodies were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. The best selected MAb combinations for quantification of human HE4 are (capture-detection respectively): Mab combination A: Cat# V100060 Clone 2- Cat# V100060 Clone 3; Mab combination B: Cat# V100060 Clone 3- Cat# V100060 Clone 1.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?