Anti-Fatty acid-binding protein antibody Mouse anti-human, monoclonal IgG1

Clinical comparison of H-FABP immunoassays and commercial diagnostic kit: 23 clinical blood samples were separately tested using AAT Bioquest’s H-FABP antibody pair on a CLIA platform. Data from this study was compared to that gathered from a commercial diagnostic kit.

Calibration curves for H-FABP in sandwich chemiluminescence immunoassay (CLIA):  anti-H-FABP (Cat #V100105, Clone 2) was coated onto 96-well microplate, and anti-H-FABP (Cat #V100105, Clone 1) was labeled with HRP. 0 to 250 ng/mL of H-FABP were tested on CLIA platform. The antibody combination used was (capture-detection respectively): MAb pair A:  Cat #V100105 (Clone 2) - Cat #V100105 (Clone 1).

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Storage, safety and handling

Intended use

Research Use Only (RUO)

Overview SDS Protocol

See also: Antibodies and Proteomics

Other names

Host

mouse

Reactivity

human

Application

ELISA; CLIA

Heart-type fatty acid-binding protein (H-FABP) is a 15 kDa cytoplasmic protein encoded by the FABP3 gene in humans. It is involved in fatty acid metabolism transporting fatty acids from the cell membrane to the mitochondria for oxidation. Following ischemic episodes, H-FABP is released from cardiac myocytes making it a sensitive biomarker for myocardial infarction. H-FABP is 20 times more specific to cardiac muscle than myoglobin and is found 10-fold lower levels in skeletal muscle than heart muscle. Anti-H-FABP monoclonal antibodies are highly sensitive for the detection of human H-FABP. They can be used for a broad range of immunoassays such as lateral-flow immunoassays (LFIA), chemiluminescent assays (CLIA) and sandwich immunoassays.

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-Fatty acid-binding protein antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

Images

Figure 1. Clinical comparison of H-FABP immunoassays and commercial diagnostic kit: 23 clinical blood samples were separately tested using AAT Bioquest’s H-FABP antibody pair on a CLIA platform. Data from this study was compared to that gathered from a commercial diagnostic kit.

Figure 2. Calibration curves for H-FABP in sandwich chemiluminescence immunoassay (CLIA): anti-H-FABP (Cat #V100105, Clone 2) was coated onto 96-well microplate, and anti-H-FABP (Cat #V100105, Clone 1) was labeled with HRP. 0 to 250 ng/mL of H-FABP were tested on CLIA platform. The antibody combination used was (capture-detection respectively): MAb pair A: Cat #V100105 (Clone 2) - Cat #V100105 (Clone 1).