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Anti-Fatty acid-binding protein antibody *Mouse anti-human, monoclonal IgG1*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application ELISA; CLIA |
Heart-type fatty acid-binding protein (H-FABP) is a 15 kDa cytoplasmic protein encoded by the FABP3 gene in humans. It is involved in fatty acid metabolism transporting fatty acids from the cell membrane to the mitochondria for oxidation. Following ischemic episodes, H-FABP is released from cardiac myocytes making it a sensitive biomarker for myocardial infarction. H-FABP is 20 times more specific to cardiac muscle than myoglobin and is found 10-fold lower levels in skeletal muscle than heart muscle. Anti-H-FABP monoclonal antibodies are highly sensitive for the detection of human H-FABP. They can be used for a broad range of immunoassays such as lateral-flow immunoassays (LFIA), chemiluminescent assays (CLIA) and sandwich immunoassays.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Fatty acid-binding protein antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
| AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
| Small Molecules | Biotin |
| Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
| iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
| mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Clinical comparison of H-FABP immunoassays and commercial diagnostic kit: 23 clinical blood samples were separately tested using AAT Bioquest’s H-FABP antibody pair on a CLIA platform. Data from this study was compared to that gathered from a commercial diagnostic kit.
Figure 2. Calibration curves for H-FABP in sandwich chemiluminescence immunoassay (CLIA): anti-H-FABP (Cat #V100105, Clone 2) was coated onto 96-well microplate, and anti-H-FABP (Cat #V100105, Clone 1) was labeled with HRP. 0 to 250 ng/mL of H-FABP were tested on CLIA platform. The antibody combination used was (capture-detection respectively): MAb pair A: Cat #V100105 (Clone 2) - Cat #V100105 (Clone 1).
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
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A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?