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AAT Bioquest

Anti-Ferritin light chain (FER) antibody *Mouse anti-human, monoclonal IgG2b*

Evaluation of anti-ferritin MAbs stability at different temperatures: anti-human ferritin monoclonal antibodies present in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days, respectively.
Evaluation of anti-ferritin MAbs stability at different temperatures: anti-human ferritin monoclonal antibodies present in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days, respectively.
Evaluation of anti-ferritin MAbs stability at different temperatures: anti-human ferritin monoclonal antibodies present in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days, respectively.
<p>Calibration curves for ferritin in sandwich chemiluminescence immunoassay (CLIA): monoclonal antibodies were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. The antigen used was human ferritin, capture MAbs was unlabeled and detection antibodies were labeled with horseradish peroxidase. The best selected MAb combinations for quantification of human ferritin are (capture-detection respectively):</p>
<p>MAb combination A: Cat# V100050 (Clone 1) - Cat# V100050 (Clone 2)</p>
<p>MAb combination D: Cat# V100050 (Clone 4) - Cat# V100050 (Clone 3)</p>
<p>&nbsp;</p>
<p>MAb combination A: Cat# V100055 (Clone 1) - Cat# V100055 (Clone 2)</p>
<p>MAb combination D: Cat# V100055 (Clone 4) - Cat# V100055 (Clone 3)</p>
Calibration curve for ferritin by LETIA: anti-human ferritin monoclonal antibodies were evaluated by latex-enhanced immunoturbidimetric assay (LETIA). A set of ferritin calibrators were reacted with specific antibodies coated onto microparticles to form insoluble complexes which were measured with a biochemical analyzer at a series of different wavelengths.
<p>Clinical comparison of CLIA Ferritin assay and commercial diagnostic assays: a study was performed where lithium heparin plasma specimens were tested in replicates of three using different anti-FER antibody combinations on a CLIA platform and compared to a commercially available diagnostic kit. MAb combination A: Cat# V100050 (Clone 1) - Cat# V100050 (Clone 2); MAb combination D: Cat# V100050 (Clone 4) - Cat# V100050 (Clone 3).</p>
<p>&nbsp;</p>
<p>MAb combination A: Cat# V100055 (Clone 1) - Cat# V100055 (Clone 2)</p>
<p>MAb combination D: Cat# V100055 (Clone 4) - Cat# V100055 (Clone 3)</p>
Comparison of LETIA ferritin assay and commercial diagnostic assays: 35 clinical blood samples were separately tested using AAT Bioquest&rsquo;s anti-FER MAbs on a LETIA platform and a commercial diagnostic kit. The correlation coefficient between the two systems is over 0.98.
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
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Physical properties
SolventWater
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C)

OverviewpdfSDSpdfProtocol


Other names
Host
mouse
Reactivity
human
Application
CLIA; LFIA; LETIA
Ferritin is a 450 kDa globular protein that stores and releases iron in a regulated fashion. It is present in most tissue types as a cytosolic protein as well as in small amounts in the serum where it serves as an iron carrier. Serum ferritin can also be used as an indirect biomarker for evaluating iron-deficiency anemia as the amount of ferritin present in serum is a direct correlation to the total amount of iron stored in the body. Anti-ferritin light chain (FER) monoclonal antibodies are highly specific for the detection of human ferritin proteins. They can be used for a broad range of immunoassays including latex enhanced turbidimetric immunoassays (LETIA), chemiluminescent immunoassays (CLIA) and rapid sandwich immunoassays.

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of Anti-Ferritin light chain (FER) antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

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