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Anti-Interleukin-6 (IL6) antibody *Mouse anti-human, monoclonal IgG1*

Comparison of IL-6 CLIA and commercial diagnostic assays: 23 samples from healthy donors and patients with chronic infections were detected using MAb combination B (Cat# V100135 (Clone 2) - Cat# V100135 (Clone 4)) in CLIA IL-6 assay and a commercial diagnostic assay. The correlation coefficient between CLIA IL-6 assay and comparison assay is over 0.97. Results show that these monoclonal antibodies can be applied to double-MAb-sandwich-immunoassays.
Comparison of IL-6 CLIA and commercial diagnostic assays: 23 samples from healthy donors and patients with chronic infections were detected using MAb combination B (Cat# V100135 (Clone 2) - Cat# V100135 (Clone 4)) in CLIA IL-6 assay and a commercial diagnostic assay. The correlation coefficient between CLIA IL-6 assay and comparison assay is over 0.97. Results show that these monoclonal antibodies can be applied to double-MAb-sandwich-immunoassays.
Comparison of IL-6 CLIA and commercial diagnostic assays: 23 samples from healthy donors and patients with chronic infections were detected using MAb combination B (Cat# V100135 (Clone 2) - Cat# V100135 (Clone 4)) in CLIA IL-6 assay and a commercial diagnostic assay. The correlation coefficient between CLIA IL-6 assay and comparison assay is over 0.97. Results show that these monoclonal antibodies can be applied to double-MAb-sandwich-immunoassays.
Six-point calibration curve for IL-6 sandwich chemiluminescence immunoassay (Calibrator concentrations: 0, 2.5, 25, 250, 750, 1500 pg/mL). All MAbs were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected MAb combinations for the development of quantitative human IL-6 immunoassays are (capture-detection respectively): MAb combination A: Cat# V100135 (Clone 1) - Cat# V100135 (Clone 4); MAb combination B: Cat# V100135 (Clone 2) - Cat# V100135 (Clone 4); MAb combination C: Cat# V100135 (Clone 3) - Cat# V100135 (Clone 4); MAb combination D: Cat# V100135 (Clone 4) - Cat# V100135 (Clone 2).
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Intended useResearch Use Only (RUO)

OverviewpdfSDSpdfProtocol


Other names
Host
mouse
Reactivity
human
Application
ELISA; CLIA; LFIA
Interleukin 6 (IL-6) is a pleotropic cytokine encoded by the IL6 gene in humans. It acts as both a pro-inflammatory cytokine and as an anti-inflammatory myokine. IL-6 is essential for stimulating acute phase protein synthesis, hybridoma growth, bone metabolism, inflammation and cancer progression. In almost all diseases involving chronic inflammation, such as diabetes rheumatoid arthritis and congestive heart failure, the concentration of IL-6 is elevated. Anti-IL-6 monoclonal antibodies are highly specific for the detection of human IL-6. They can be used for a broad range of immunoassays such as chemiluminescent immunoassays (CLIA) and rapid sandwich immunoassays.

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of Anti-Interleukin-6 (IL6) antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

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