Anti-Myeloperoxidase (MPO) antibody Mouse anti-human, monoclonal IgG2a

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C)

Overview SDS Protocol

See also: Antibodies and Proteomics

Other names

Host

mouse

Reactivity

human

Application

LETIA; ELISA; CLIA; LFIA

Myeloperoxidase (MPO) is a 150 kDa cationic hemoprotein that consists of two covalently bound subunits each containing a heavy chain (60 kDa) and a light chain (15 kDa). MPO is abundantly expressed in neutrophils and is released during degranulation. It plays a vital role in inflammatory responses by catalyzing chloride ion oxidation to produce hypochlorous acid which is used by neutrophils to kill bacteria and other pathogens. MPO is also known to cause oxidative modification of low density lipoproteins to a high uptake form. This is considered to be a key event in the promotion of atherogenesis and is linked to the progression of cardiovascular diseases. As such, MPO is considered to be a promising cardiovascular biomarker as elevated MPO levels indicates a high risk for atherosclerosis and coronary heart disease (CHD). Anti-MPO monoclonal antibodies are highly sensitive for the detection of human MPO. They can be used for a broad range of immunoassays such as latex enhanced turbidimetric immunoassays (LETIA), chemiluminescent immunoassays (CLIA) and ELISA.

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-Myeloperoxidase (MPO) antibody *Mouse anti-human, monoclonal IgG2a* with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

Images

Figure 1. Samples from healthy donors and patients with myocardial injury were detected using the chemiluminescence immunoassay (CLIA) MPO assays, A and B. Results show good correlation between CLIA MPO assays and comparison kits.

Figure 2. Calibration curve for MPO in latex enhanced turbidimetric immunoassay (LETIA): human MPO proteins were reacted with anti-human MPO antibodies coated onto latex microspheres, resulting in agglutination and an increase in turbidity. Changes in absorbance were monitored using a spectrometer to quantitatively measure the MPO concentration in the samples.

Figure 3. Calibration curve for MPO in chemiluminescent immunoassay (CLIA) using the best two-site MAb combination for the quantitative detection of MPO. A double monoclonal antibody sandwich method was used using Cat# V100095 (Clone 1) as capture antibodies and Cat# V100095 (Clone 2) labeled with horseradish peroxidase (HRP) as detection antibodies. The calibration curve between MPO concentration and relative light units was analyzed by four-parameter logistic (4PL) regression model (R²=0.9996).

Figure 4. Shows the comparison of MPO concentrations determined using AAT Bioquest’s anti-MPO monoclonal antibodies and two high-quality kits (A and B). Anti-MPO monoclonal antibodies were evaluated by immunoturbidimetric assay in medium-scale clinical trials with random blood samples from donors (n=72). Results reveal good agreement between AAT Bioquest’s immunoassay and comparison assays.