Anti-Myeloperoxidase (MPO) antibody *Mouse anti-human, monoclonal IgG2b*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Overview | SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application LETIA; ELISA; CLIA; LFIA |
Myeloperoxidase (MPO) is a 150 kDa cationic hemoprotein that consists of two covalently bound subunits each containing a heavy chain (60 kDa) and a light chain (15 kDa). MPO is abundantly expressed in neutrophils and is released during degranulation. It plays a vital role in inflammatory responses by catalyzing chloride ion oxidation to produce hypochlorous acid which is used by neutrophils to kill bacteria and other pathogens. MPO is also known to cause oxidative modification of low density lipoproteins to a high uptake form. This is considered to be a key event in the promotion of atherogenesis and is linked to the progression of cardiovascular diseases. As such, MPO is considered to be a promising cardiovascular biomarker as elevated MPO levels indicates a high risk for atherosclerosis and coronary heart disease (CHD). Anti-MPO monoclonal antibodies are highly sensitive for the detection of human MPO. They can be used for a broad range of immunoassays such as latex enhanced turbidimetric immunoassays (LETIA), chemiluminescent immunoassays (CLIA) and ELISA.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Myeloperoxidase (MPO) antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Samples from healthy donors and patients with myocardial injury were detected using the chemiluminescence immunoassay (CLIA) MPO assays, A and B. Results show good correlation between CLIA MPO assays and comparison kits.
Figure 2. Calibration curve for MPO in latex enhanced turbidimetric immunoassay (LETIA): human MPO proteins were reacted with anti-human MPO antibodies coated onto latex microspheres, resulting in agglutination and an increase in turbidity. Changes in absorbance were monitored using a spectrometer to quantitatively measure the MPO concentration in the samples.
Figure 3. Calibration curve for MPO in chemiluminescent immunoassay (CLIA) using the best two-site MAb combination for the quantitative detection of MPO. A double monoclonal antibody sandwich method was used using Cat# V100095 (Clone 1) as capture antibodies and Cat# V100095 (Clone 2) labeled with horseradish peroxidase (HRP) as detection antibodies. The calibration curve between MPO concentration and relative light units was analyzed by four-parameter logistic (4PL) regression model (R2=0.9996).
Figure 4. Shows the comparison of MPO concentrations determined using AAT Bioquest’s anti-MPO monoclonal antibodies and two high-quality kits (A and B). Anti-MPO monoclonal antibodies were evaluated by immunoturbidimetric assay in medium-scale clinical trials with random blood samples from donors (n=72). Results reveal good agreement between AAT Bioquest’s immunoassay and comparison assays.
Application notes
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A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
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What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?