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AAT Bioquest

Anti-Neutrophil gelatinase-associated lipocalin (NGAL) antibody *Mouse anti-human, monoclonal IgG2b*

Evaluation of the relative titers of four anti-NGAL MAbs stored at different temperature. AAT Bioquest’s anti-human NGAL monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days. During this period, the titers of four MAbs were determined in 3 day intervals.
Evaluation of the relative titers of four anti-NGAL MAbs stored at different temperature. AAT Bioquest’s anti-human NGAL monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days. During this period, the titers of four MAbs were determined in 3 day intervals.
Evaluation of the relative titers of four anti-NGAL MAbs stored at different temperature. AAT Bioquest’s anti-human NGAL monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days. During this period, the titers of four MAbs were determined in 3 day intervals.
<p>Calibration curves for NGAL in sandwich chemiluminescence immunoassay (CLIA): all monoclonal antibodies were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. The best selected MAb combinations for quantification of human NGAL are (capture-detection): MAb combination A: Cat# V100000 (Clone 1) &ndash; Cat# V100005 (Clone 1); MAb combination B: Cat# V100005 (Clone 1) - Cat# V100000 (Clone 1); MAb combination C: Cat# V100005 (Clone 2) - Cat# V100000 (Clone 1); and MAb combination D: Cat# V100005 (Clone 3) - Cat# V100000 (Clone 1).</p>
<p>&nbsp;</p>
<p>MAb combination A: CSB-DA001DmNâ‘  - CSB-DA001AmNâ‘ </p>
<p>MAb combination B: CSB-DA001AmNâ‘  - CSB-DA001DmNâ‘ </p>
<p>MAb combination C: CSB-DA001AmNâ‘¡ - CSB-DA001DmNâ‘ </p>
<p>MAb combination D: CSB-DA001AmNâ‘¢ - CSB-DA001DmNâ‘ </p>
Calibration curve for human NGAL in latex-enhanced turbidimetric immunoassay: a set of NGAL calibrators were reacted with specific antibodies coated onto microparticles to form insoluble complexes that were measured turbidimetrically at a series of different wavelengths.
Determination of clinical&nbsp;samples using four MAb combinations on CLIA platform. A study was performed where 50 specimens were tested using different antibody combinations (A, B, C and D) on AAT Bioquest&rsquo;s CLIA platform and compared to a high-quality competitor&rsquo;s assay. Results showed the four combinations have correlation coefficients (r) &gt; 0.90. The MAb combinations used are (capture-detection): MAb combination A: Cat# V100000 (Clone 1) &ndash; Cat# V100005 (Clone 1); MAb combination B: Cat# V100005 (Clone 1) - Cat# V100000 (Clone 1); MAb combination C: Cat# V100005 (Clone 2) - Cat# V100000 (Clone 1); and MAb combination D: Cat# V100005 (Clone 3) - Cat# V100000 (Clone 1).
In order to meet the application of AAT Bioquest&rsquo;s anti-NGAL MAbs on LETIA platform, 40 clinical blood samples were separately tested using mixed MAbs on AAT Bioquest&rsquo;s LETIA platform and compared to a competitor&rsquo;s diagnostic kit. The correlation coefficient between the two systems is over 0.90.
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Intended useResearch Use Only (RUO)

OverviewpdfSDSpdfProtocol


Other names
Host
mouse
Reactivity
human
Application
ELISA; CLIA; LFIA
Neutrophil gelatinase-associated lipocalin (NGAL) is a protein expressed mostly in neutrophils. It is also present in lower levels in the kidney, prostate and epithelia of the respiratory and alimentary tracts. NGAL is a vital component in the innate immune response to bacterial infection. It limits bacterial growth by sequestering iron-containing microorganisms. NGAL's small size and protease resistant properties makes it easily excreted and detected in the urine. Under normal conditions, NGAL levels in the urine and plasma are low. However, in response to kidney injury NGAL levels increase sharply reaching diagnostic levels quickly. In clinical biology, NGAL is a novel biomarker of acute kidney injury (AKI) and is used in the early diagnosis of chronic kidney disease. Anti-NGAL monoclonal antibodies are highly sensitive for the detection of human NGAL. They can be used for a broad range of immunoassays such as chemiluminescent immunoassays (CLIA), latex enhanced turbidimetric immunoassays (LETIA), lateral-flow immunoassays (LFIA) and ELISA.

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of Anti-Neutrophil gelatinase-associated lipocalin (NGAL) antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

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