Anti-Platelet-activating factor acetylhydrolase (Lp-PLA2) antibody Mouse anti-human, monoclonal IgG1

Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.

<p>Calibration curve for Lp-PLA2 sandwich chemiluminescence immunoassay (CLIA): all MAbs were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected MAb combinations for the development of quantitative human Lp-PLA2 immunoassays are (capture-detection): MAb combination A:  Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C:  Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E:  Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).</p>
<p> </p>
<p>MAb combination A:  Cat# V100090 (Clone 1) - Cat# V100090 (Clone 3); MAb combination C:  Cat# V100090 (Clone 3) - Cat# V100090 (Clone 1); MAb combination E:  Cat# V100090 (Clone 4) - Cat# V100090 (Clone 5).</p>
<p> </p>
<p>MAb combination A:  Cat# V100085 (Clone 1) - Cat# V100085 (Clone 3); MAb combination C:  Cat# V100085 (Clone 3) - Cat# V100085 (Clone 1); MAb combination E:  Cat# V100085 (Clone 4) - Cat# V100085 (Clone 5).</p>

Determination of clinical samples on CLIA using MAb combinations: 81 clinical samples from healthy donors and patients with atherosclerosis or cerebral infarction were detected using various Mab combinations in a CLIA Lp-PLA2 assay and a competitor’s assay. The Mab combinations used are (capture-detection): MAb combination A:  Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C:  Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E:  Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Intended use

Research Use Only (RUO)

Overview SDS Protocol

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of Anti-Platelet-activating factor acetylhydrolase (Lp-PLA2) antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

Images

Figure 1. Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.

Figure 2.

Calibration curve for Lp-PLA2 sandwich chemiluminescence immunoassay (CLIA): all MAbs were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected MAb combinations for the development of quantitative human Lp-PLA2 immunoassays are (capture-detection): MAb combination A: Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C: Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E: Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).

MAb combination A: Cat# V100090 (Clone 1) - Cat# V100090 (Clone 3); MAb combination C: Cat# V100090 (Clone 3) - Cat# V100090 (Clone 1); MAb combination E: Cat# V100090 (Clone 4) - Cat# V100090 (Clone 5).

MAb combination A: Cat# V100085 (Clone 1) - Cat# V100085 (Clone 3); MAb combination C: Cat# V100085 (Clone 3) - Cat# V100085 (Clone 1); MAb combination E: Cat# V100085 (Clone 4) - Cat# V100085 (Clone 5).

Figure 3. Determination of clinical samples on CLIA using MAb combinations: 81 clinical samples from healthy donors and patients with atherosclerosis or cerebral infarction were detected using various Mab combinations in a CLIA Lp-PLA2 assay and a competitor’s assay. The Mab combinations used are (capture-detection): MAb combination A: Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C: Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E: Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).