Anti-Platelet-activating factor acetylhydrolase (Lp-PLA2) antibody *Mouse anti-human, monoclonal IgG2b*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Overview | SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application LETIA; ELISA; CLIA; LFIA |
Lipoprotein-associated phospholipase A2 (Lp-PLA2), or platelet-activating factor acetylhydrolase (PAF-AH), is a 45 kDa phospholipase enzyme produced by inflammatory cells such as mature macrophages and activated platelets. Lp-PLA2 is typically found present in the blood coupled to lipoproteins: 80% to LDL and 20% to HDL. It is essential for the hydrolysis of oxidized phospholipids, yielding pro-inflammatory lysophospholipids and oxidized fatty acids. In clinically biology, Lp-PLA2 is a significant biomarker associated with the development of atherosclerosis and cardiac disease. Anti-Lp-PLA2 monoclonal antibodies are highly specific for the detection of human Lp-PLA2 immunogens. They can be used for a broad range of immunoassays such as chemiluminescent immunoassay (CLIA), latex enhanced turbidimetric immunoassay (LETIA), ELISA and rapid sandwich immunoassays.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Platelet-activating factor acetylhydrolase (Lp-PLA2) antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.
Figure 2. Calibration curve for Lp-PLA2 sandwich chemiluminescence immunoassay (CLIA): all MAbs were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected MAb combinations for the development of quantitative human Lp-PLA2 immunoassays are (capture-detection): MAb combination A: Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C: Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E: Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).
MAb combination A: Cat# V100090 (Clone 1) - Cat# V100090 (Clone 3); MAb combination C: Cat# V100090 (Clone 3) - Cat# V100090 (Clone 1); MAb combination E: Cat# V100090 (Clone 4) - Cat# V100090 (Clone 5).
MAb combination A: Cat# V100085 (Clone 1) - Cat# V100085 (Clone 3); MAb combination C: Cat# V100085 (Clone 3) - Cat# V100085 (Clone 1); MAb combination E: Cat# V100085 (Clone 4) - Cat# V100085 (Clone 5).
Figure 3. Determination of clinical samples on CLIA using MAb combinations: 81 clinical samples from healthy donors and patients with atherosclerosis or cerebral infarction were detected using various Mab combinations in a CLIA Lp-PLA2 assay and a competitor’s assay. The Mab combinations used are (capture-detection): MAb combination A: Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C: Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E: Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
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Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?