Anti-Platelet-activating factor acetylhydrolase (Lp-PLA2) antibody *Mouse anti-human, monoclonal IgG3*
Lipoprotein-associated phospholipase A2 (Lp-PLA2), or platelet-activating factor acetylhydrolase (PAF-AH), is a 45 kDa phospholipase enzyme produced by inflammatory cells such as mature macrophages and activated platelets. Lp-PLA2 is typically found present in the blood coupled to lipoproteins: 80% to LDL and 20% to HDL. It is essential for the hydrolysis of oxidized phospholipids, yielding pro-inflammatory lysophospholipids and oxidized fatty acids. In clinically biology, Lp-PLA2 is a significant biomarker associated with the development of atherosclerosis and cardiac disease. Anti-Lp-PLA2 monoclonal antibodies are highly specific for the detection of human Lp-PLA2 immunogens. They can be used for a broad range of immunoassays such as chemiluminescent immunoassay (CLIA), latex enhanced turbidimetric immunoassay (LETIA), ELISA and rapid sandwich immunoassays.
![Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3%2Ffigure-for-anti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3_SwLqU.jpg&w=640&q=75)
![Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3%2Ffigure-for-anti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3_SwLqU.jpg&w=640&q=75)
![Thermal stability profiles of four monoclonal antibodies: anti-human Lp-PLA2 monoclonal antibodies presented in PBS buffer (without any preservative) were stored at -20°C, 2-8°C and 37°C for 15 days.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3%2Ffigure-for-anti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3_SwLqU.jpg&w=128&q=25)
![<p>Calibration curve for Lp-PLA2 sandwich chemiluminescence immunoassay (CLIA): all MAbs were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected MAb combinations for the development of quantitative human Lp-PLA2 immunoassays are (capture-detection): MAb combination A: Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C: Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E: Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).</p>
<p> </p>
<p>MAb combination A: Cat# V100090 (Clone 1) - Cat# V100090 (Clone 3); MAb combination C: Cat# V100090 (Clone 3) - Cat# V100090 (Clone 1); MAb combination E: Cat# V100090 (Clone 4) - Cat# V100090 (Clone 5).</p>
<p> </p>
<p>MAb combination A: Cat# V100085 (Clone 1) - Cat# V100085 (Clone 3); MAb combination C: Cat# V100085 (Clone 3) - Cat# V100085 (Clone 1); MAb combination E: Cat# V100085 (Clone 4) - Cat# V100085 (Clone 5).</p>](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3%2Ffigure-for-anti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3_y5GNJ.jpg&w=128&q=25)
![Determination of clinical samples on CLIA using MAb combinations: 81 clinical samples from healthy donors and patients with atherosclerosis or cerebral infarction were detected using various Mab combinations in a CLIA Lp-PLA2 assay and a competitor’s assay. The Mab combinations used are (capture-detection): MAb combination A: Cat# V100080 (Clone 1) - Cat# V100080 (Clone 3); MAb combination C: Cat# V100080 (Clone 3) - Cat# V100080 (Clone 1); MAb combination E: Cat# V100080 (Clone 4) - Cat# V100080 (Clone 5).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3%2Ffigure-for-anti-platelet-activating-factor-acetylhydrolase-lp-pla2-antibody-mouse-anti-human-monoclonal-igg3_i1onZ.jpg&w=96&q=25)
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Platelet-activating factor acetylhydrolase (Lp-PLA2) antibody *Mouse anti-human, monoclonal IgG3* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.