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Anti-Prolactin (PRL) antibody *Mouse anti-human, monoclonal IgG2b*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application ELISA; CLIA |
Prolactin (PRL), also known as luteotropic hormone, is a protein secreted from the pituitary gland. It plays a vital role in metabolism, immune system regulation and pancreatic development, but is best known for its role in producing milk in mammals. In normal individuals, PRL concentrations increase in response to physiologic stimuli such as eating, mating, exercise, stress and hypoglycemia. PRL levels are also elevated during pregnancy, lactation, postpartum and in newborn infants. In clinical biology, prolactin tests are used in the diagnosis of prolactinoma, a prolactin-producing tumor of the pituitary gland. Anti-PRL monoclonal antibodies are highly sensitive for the detection of human purified prolactin. They can be used for a broad range of immunoassays such as chemiluminescent immunoassays (CLIA) and in the development of highly sensitive sandwich immunoassays.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Prolactin (PRL) antibody *Mouse anti-human, monoclonal IgG2b* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
| AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
| Small Molecules | Biotin |
| Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
| iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
| mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Clinical comparison of AAT Bioquest’s prolactin immunoassays and the Access Prolactin assay from Beckman Coulter: 80 clinical blood samples were separately tested using MAb combination A and B on AAT Bioquest’s CLIA platform and compared to a diagnostic kit from Beckman Coulter. Data from this study were analyzed using the Passing-Bablok regression method and are summarized in the following scatter plot. Results show good agreement between AAT Bioquest’s immunoassays and comparison assays.
Figure 2. Calibration curves for prolactin in sandwich chemiluminescence immunoassay (CLIA). All monoclonal antibodies were tested in pairs as capture and detection antibodies to determine the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected MAb combinations for quantification of human prolactin are (capture-detection): MAb combination A: Cat# V100160 (Clone 1) - Cat# V100160 (Clone 2)ï¼›MAb combination B: Cat# V100160 (Clone 1) - Cat# V100160 (Clone 3).
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
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A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
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What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
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What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?