Anti-Retinol-binding protein 4 (RBP4) antibody *Mouse anti-human, monoclonal IgG1*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Overview | SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application ELISA; LETIA |
Retinol binding protein 4 (RBP4) is a protein belonging to the lipocalin family. It functions primarily as a carrier for retinol (vitamin A) delivering it from liver stores to peripheral tissues. In aqueous solutions, retinol is insoluble and quite unstable. When in plasma, retinols solubility and stability improve due to its tight interaction with RBP4. It is also here that RBP4-retinol complexes interact with transthyretin (TTR), a thyroxine-binding protein. This interaction yields retinol-RBP4-TTR complexes which prevents the loss of RBP4 by filtration through the kidney glomeruli. In recent years, mutations in the RBP4 gene have been linked to a form of autosomal dominant microphthalmia, anophthalmia, and coloboma (MAC) disease, making it a promising therapeutic target for the treatment of MAC. Anti-RBP4 monoclonal antibodies are highly sensitive for the detection of human RBP4 immunogens. They are well-suited for use in immunoassays such as in latex enhanced turbidity immunoassays (LETIA).
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Retinol-binding protein 4 (RBP4) antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Determination of serum RBP4 using reagents made with AAT Bioquest’s RBP4 monoclonal antibodies and a competitor’s assays. Mouse anti-human RBP4 monoclonal antibodies were evaluated in medium-scale clinical tries with blood samples.
Figure 2. Calibration curve for RBP4 in latex-enhanced turbidimetric immunoassay (LETIA): RBP4 proteins were reacted with anti-RBP4 monoclonal antibodies pre-coated onto latex beads to form insoluble complexes, resulting in turbidity increasing. Changes in absorbance were detected using an automatic biochemical analyzer.
Figure 3. Determination of the RBP4 concentration by serial dilution of clinical serum: the high-value RBP4 serum was serially diluted two-fold with physiological saline, and then measured using our LETIA platform. The measured RBP4 concentrations gradually declined along with the serial dilution of blood samples.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?