Anti-Thyroglobulin antibody *Mouse anti-human, monoclonal IgG1*
Ordering information
Price | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Overview | SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application ELISA; CLIA |
Thyroglobulin (TG) is a 660 kDa glycoprotein homodimer produced by follicular cells of the thyroid gland. It is used predominately within the thyroid gland as a precursor for the synthesis of thyroxine and triiodothyronine, both of which are thyroid hormones essential for regulating metabolism. Under normal physiological conditions, the levels of thyroglobulin circulating in the blood stream are low. However, in any thyroid pathology the level of thyroglobulin increases making it a promising biomarker for thyroid cancers and thyroid diseases such as Graves' disease. Anti-TG monoclonal antibodies are highly sensitive for the detection of human thyroglobulin immunogens. They can be used for a broad range of immunoassays such as chemiluminescent immunoassays (CLIA), and for IVD assay development.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Thyroglobulin antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Comparison between AAT Bioquest’s CLIA TG immunoassay and a competitor’s immunoassay: samples from donors were detected using different MAb combinations (A and B) from AAT Bioquest on a CLIA TG immunoassay, and compared to a competitor’s immunoassay. Results showed good correlation between both systems. The MAb combinations used are (capture-detection): Mab combination A: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 2); and Mab combination B: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 3).
Figure 2. Calibration curve for TG in chemiluminescence immunoassay (CLIA): all MAbs were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP).The best selected MAb combinations for the development of quantitative human TG immunoassays are (capture-detection): Mab combination A: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 2); and Mab combination B: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 3).
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
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A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?