APC Anti-human CD116 Antibody 4H1

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (280 nm)	0.195
Extinction coefficient (cm ^-1 M ^-1)	730000
Excitation (nm)	651
Emission (nm)	660

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

GM-CSFRα chain

Clone

4H1

Host

mouse

Isotype

Mouse IgG1 kappa

Reactivity

human

Application

Correction Factor (280 nm)

0.195

Extinction coefficient (cm ^-1 M ^-1)

730000

Excitation (nm)

651

Emission (nm)

660

The 4H1 monoclonal antibody binds to human CD116, a 70 - 85 kD glycoprotein commonly located on the surface of endothelial precursors and macrophages. CD116 is associated with a variety of biologically interesting macromolecules/ligands, for instance, CD131 and GM-CSF. CD116 is a relatively rare antibody target, with fewer than 100 publications in the last decade. Even still, CD116 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology. This antibody was purified through affinity chromatography and conjugated to APC (ex/em = 651/660 nm). It is compatible with the 642 nm laser and 702/87 nm bandpass filter (for example, as in the Luminex Amnis CellStream).

Spectrum

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Spectral properties

Correction Factor (280 nm)	0.195
Extinction coefficient (cm ^-1 M ^-1)	730000
Excitation (nm)	651
Emission (nm)	660

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd116 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780