APC Anti-human CD27 Antibody *LT27*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (280 nm) | 0.195 |
Extinction coefficient (cm -1 M -1) | 730000 |
Excitation (nm) | 651 |
Emission (nm) | 660 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names T14; S152; TNFRSF7 | Clone LT27 | Host mouse | Isotype Mouse IgG2a | Reactivity human | Application | Correction Factor (280 nm) 0.195 | Extinction coefficient (cm -1 M -1) 730000 | Excitation (nm) 651 | Emission (nm) 660 |
LT27 is an anti-human monoclonal antibody that recognizes the CD27 antigen. CD27 (sometimes referred to as T14, S152 or TNFRSF7) is a 50 - 55 kD member of the TNF-R superfamily that is expressed on the surface of cells such as NK cells, B cells and T cells. In some organisms, CD27 is a promoter of T cell differentiation, acts to positively regulate NIK/NF-kappaB signaling and is involved in the negative regulation of apoptotic process. Furthermore, it plays a role in key cellular pathways, in particular, the tumor necrosis factor-mediated signaling pathway, cell surface receptor signaling pathway and extrinsic apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like TRAF5, TRAF2 and CD70. CD27 is a moderately popular antibody target, with over 11000 publications in the last decade. CD27 is essential for immunology and costimulatory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to APC (ex/em = 651/660 nm). It is compatible with the 642 nm laser and 702/85 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (280 nm) | 0.195 |
Extinction coefficient (cm -1 M -1) | 730000 |
Excitation (nm) | 651 |
Emission (nm) | 660 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd27 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?