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APC Anti-human CD279 Antibody *2E7*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
PD1
Clone
2E7
Host
mouse
Reactivity
human
Application
Correction Factor (280 nm)
0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)
651
Emission (nm)
660
2E7 is an anti-human monoclonal antibody that targets the CD279 antigen. CD279 (sometimes referred to as Programmed Death-1 or PD-1) is a 50 - 55 kD glycoprotein that is found on the surface of cells like B cells and T cells. CD279 is associated with a variety of biologically interesting macromolecules/ligands, namely, PDL1. CD279 is a relatively rare antibody target, with less than 1000 publications in the last decade. Even still, CD279 has been widely used in cancer biomarkers, immunology and inhibitory molecules research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to APC (ex/em = 651/660 nm). It is compatible with the 642 nm laser and 702/85 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)730000
Excitation (nm)651
Emission (nm)660

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd279 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.