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APC Anti-human CD4 Antibody
SK3
SK3 is an anti-human monoclonal antibody that recognizes the CD4 antigen. CD4 (alternatively called T4 or Leu3a) is a 55 kD member of the Ig superfamily that is located on the surface of cells such as macrophages, T cells and granulocytes. CD4 has been associated with important biological processes such as membrane organization, specifically fusion of virus membrane with host plasma membrane. Furthermore, it is involved with vital cellular pathways, for example, the T cell receptor signaling pathway, enzyme linked receptor protein signaling pathway and cytokine-mediated signaling pathway. In some organisms, CD4 acts to positively regulate peptidyl-tyrosine phosphorylation, is an enhancer of transcription, DNA-templated and is a positive regulator of ERK1 and ERK2 cascade. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as MHC Class II, gp120, IL-16 and Lck. CD4 is a very popular antibody target, with over 180000 publications in the last decade. CD4 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to APC (ex/em = 651/660 nm). It is compatible with the 642 nm laser and 702/87 nm bandpass filter (for example, as in the Luminex Amnis CellStream).
Detection of CD4 expression on human peripheral blood lymphocytes stained by flow cytometry. Human PBMCs were stained with APC anti-human CD4 monoclonal antibody *SK3* (Cat No. 100421C1). The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the APC channel.
Detection of CD4 expression on human peripheral blood lymphocytes stained by flow cytometry. Human PBMCs were stained with APC anti-human CD4 monoclonal antibody *SK3* (Cat No. 100421C1). The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the APC channel.
protocol
Protocol
COA
CatalogSize
Price
Quantity
100421C025 tests
Price
100421C1100 tests
Price
100421C2500 tests
Price
 
Antibody properties
Other namesLeu-3; T4
CloneSK3
Hostmouse
IsotypeMouse IgG1, κ
Reactivityhuman
Spectral properties
Correction factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on September 23, 2025