APC Anti-human CD45 Antibody *HI100*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Extinction coefficient (cm -1 M -1) | 700000 |
Excitation (nm) | 651 |
Emission (nm) | 660 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | ![]() ![]() |
Other names PTPRC | Clone HI100 | Host mouse | Isotype Mouse IgG2b, κ | Reactivity human | Application | Extinction coefficient (cm -1 M -1) 700000 | Excitation (nm) 651 | Emission (nm) 660 |
HI100 is an anti-human monoclonal antibody that recognizes the CD45ra antigen. CD45ra (sometimes referred to as LY5, PTPRC, GP180 or LCA) is a 205 - 220 kD glycoprotein that is located on the surface of cells such as T cells and B cells. CD45 has been thought to be involved with essential biological processes like dephosphorylation, particularly protein dephosphorylation. Additionally, in certain organisms, it represses microglial cell activation, is involved in the positive regulation of humoral immune response mediated by circulating immunoglobulin and plays a role in the upregulation of extrinsic apoptotic signaling pathway. CD45 is a member of important cellular pathways, in particular, the cell surface receptor signaling pathway, positive regulation of antigen receptor-mediated signaling pathway and T cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as Src kinases. CD45 is a very popular antibody target, with over 54000 publications in the last decade. CD45ra is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of neuroscience, inhibitory molecules and immunology. This antibody was purified through affinity chromatography and conjugated to APC (ex/em = 651/660 nm). It is compatible with the 642 nm laser and 664/20 nm bandpass filter (for example, as in the Luminex Guava easyCyte).
Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 700000 |
Excitation (nm) | 651 |
Emission (nm) | 660 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd45ra antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?