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APC Anti-human CD7 Antibody
HIT7
HIT7 is an anti-human monoclonal antibody that recognizes the CD7 antigen. CD7 (also known as gp40) is a 40 kD single-pass type I membrane protein that is located on the surface of cells like NK cells, stem cells and T cells. CD7 has been closely linked to essential biological processes such as immune response, particularly adaptive immune response. Furthermore, it is involved with important cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like PI3-Kinase. CD7 is a fairly uncommon antibody target, with a little more than 3600 publications in the last decade. Even still, CD7 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to APC (ex/em = 651/660 nm). It is compatible with the 642 nm laser and 702/85 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with APC Anti-human CD7 Antibody *HIT7*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific R1-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with APC Anti-human CD7 Antibody *HIT7*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific R1-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
100701C025 tests
Price
100701C1100 tests
Price
100701C2500 tests
Price
 
Antibody properties
Other namesgp40, TP41
CloneHIT7
HostMouse
IsotypeMouse IgG1
ReactivityHuman
Spectral properties
Correction factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on December 21, 2025