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AAT Bioquest

APC Anti-mouse CD197 Antibody *4B12*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
CCR7; BLR2; EBI1; CMKBR7
Clone
4B12
Host
rat
Isotype
Rat IgG2a kappa
Reactivity
mouse
Application
Correction Factor (280 nm)
0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)
651
Emission (nm)
660
4B12 is an anti-mouse monoclonal antibody that is specific for the CD197 antigen. CD197 (sometimes called BLR2, CCR7, EBI1 or CMKBR7) is a glycoprotein that is expressed on the surface of cells like B cells, T cells and dendritic cells. CD197 is associated with a variety of biologically interesting macromolecules/ligands, for example, CCL19/ECL and CCL21. CD197 is a relatively rare antibody target, with fewer than 500 publications in the last decade. Even still, CD197 is essential for immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to APC (ex/em = 651/660 nm). It is compatible with the 642 nm laser and 664/20 nm bandpass filter (for example, as in the Luminex Guava easyCyte).

Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)730000
Excitation (nm)651
Emission (nm)660

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd197 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.