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APC/Cy7 Anti-human CD3 Antibody
SK7
The SK7 monoclonal antibody reacts with human CD3e, a 20 kD member of the Ig superfamily often found on the surface of t cells, tregs and nkt cells. In many organisms, CD3 positively regulates interleukin-2 biosynthetic process, enhances cell-matrix adhesion and is a positive regulator of gene expression. Moreover, it plays a role in essential cellular pathways, for example, the T cell receptor signaling pathway, negative regulation of smoothened signaling pathway and apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to APC/Cy7 (ex/em = 754/779 nm).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with APC/Cy7 Anti-human CD3 Antibody *SK7*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific R7-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with APC/Cy7 Anti-human CD3 Antibody *SK7*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific R7-A channel.
datasheet
Datasheet
sds
SDS
COA
CatalogSize
Price
Quantity
100331D025 tests
Price
100331D1100 tests
Price
100331D2500 tests
Price
 
Antibody properties
Other namesT3E
CloneSK7
HostMouse
ReactivityHuman
Spectral properties
Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)651
Emission (nm)779
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on February 25, 2026