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AAT Bioquest

APC/iFluor® 700 Anti-human CD19 Antibody *HIB19*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)710
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
B4
Clone
HIB19
Host
mouse
Reactivity
human
Application
Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)
651
Emission (nm)
710
HIB19 is an anti-human monoclonal antibody that recognizes the CD19 antigen. CD19 (also known as CVID3) is a 95 kD glycoprotein that is located on the surface of cells like stem cells, dendritic cells and B cells. In certain organisms, CD19 promotes release of sequestered calcium ion into cytosol, plays a role in the upregulation of phosphatidylinositol 3-kinase activity and acts to positively regulate protein kinase B signaling. In addition, it is a member of critical cellular pathways, for example, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as lyn. CD19 is a very popular antibody target, with over 36000 publications in the last decade. CD19 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to APC/iFluor® 700 (ex/em = 685/710 nm).

Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)710

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd19 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.