Name: APC/XFD750 Anti-human CD25 Antibody *HI25a*
Brand: AAT Bioquest
SKU: 102501E0
Availability: InStock

APC/XFD750 Anti-human CD25 Antibody

HI25a

HI25a is an anti-human monoclonal antibody that is specific for the CD25 antigen. CD25 (sometimes called IL2RA) is a 55 kD transmembrane protein that is found on the surface of cells such as T cells, macrophages, B cells and NK cells. In certain organisms, CD25 is involved in the positive regulation of activated T cell proliferation, is a negative regulator of T cell proliferation and plays a role in the upregulation of T cell differentiation. Additionally, it is a component of important cellular pathways, for example, the cytokine-mediated signaling pathway, interleukin-2-mediated signaling pathway and Notch signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like IL-2. CD25 is a very popular antibody target, with over 40000 publications in the last decade. CD25 has a variety of applications in immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to APC/XFD750 (ex/em = 756/785 nm). XFD750 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 750 (Alexa Fluor® is the trademark of Thermo Fisher).

Datasheet

COA

Catalog	Size	Price
102501E0	25 tests	Price
102501E1	100 tests	Price
102501E2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD25 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	IL-2Rα, p55, TAC antigen
Clone	HI25a
Host	Mouse
Isotype	IgG1
Reactivity	Human

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	785

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on July 29, 2026