ATTO 514 PEG4 DBCO

• Ex/Em: 510/531 nm
• Extinction coefficient: 115,000 cm^-1M^-1
• Reactive group: DBCO
• Copper-Free Bioorthogonal Labeling: Enables efficient azide conjugation via SPAAC without copper, ideal for sensitive systems
• Superior Brightness & Stability: Provides robust quantum yield with high photostability and thermal stability
• Excellent Hydrophilicity: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

ATTO 514 PEG4 DBCO is manufactured by AAT Bioquest for research and development use. ATTO 514 is a hydrophilic fluorescent dye characterized by excellent aqueous solubility, strong molar absorptivity, high fluorescence quantum yield, and notable thermal and photostability. These properties make it well-suited for advanced imaging techniques, including single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. ATTO 514 is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other fluorescence-based assays. The dye is optimally excited within the 510–535 nm range, with the 514 nm line of an Argon-Ion laser providing efficient excitation.

The DBCO derivative of ATTO 514 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, ATTO 514 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.