ATTO 532 PEG4 DBCO

• Ex/Em: 531/552 nm
• Extinction coefficient: 115,000 cm^-1M^-1
• Reactive group: DBCO
• Click Chemistry: enables copper-free, bioorthogonal labeling of azide-functionalized biomolecules
• High Quantum Yield & Photostability: ensures intense fluorescence signals for reliable detection and imaging
• Excellent Hydrophilicity: Prevents aggregation and enhances signal clarity in live-cell applications
• Advanced Imaging Performance: Suitable for single-molecule detection and super-resolution techniques, including SIM and STED

ATTO 532 PEG4 DBCO is manufactured by AAT Bioquest for research and development use. ATTO 532 is a rhodamine-based fluorescent dye notable for its high molar absorptivity and fluorescence quantum yield (0.90), providing robust signal intensity in fluorescence applications. Its combination of photostability, aqueous solubility, and sufficient Stokes shift makes it suitable for single-molecule detection and high-resolution microscopy techniques, including SIM and STED microscopy. ATTO 532 is also effective in flow cytometry, FISH, and a variety of biological assays, offering flexibility for diverse fluorescence-based experimental protocols. The dye is optimally excited within the 515-545 nm range, with a frequency-doubled Nd:YAG laser at 532 nm serving as an ideal excitation source.

The DBCO derivative of ATTO 532 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, ATTO 532 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.