ATTO 565 azide

• Ex/Em: 562/589 nm
• Extinction coefficient: 120,000 cm^-1M^-1
• Reactive group: azide
• Precise Conjugation: Readily and selectively labels alkyne targets via click chemistry
• High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
• Enhanced Signal Separation: Provides effective signal clarity by minimizing background interference

ATTO 565 azide is manufactured by AAT Bioquest for research and development use. ATTO 565 is a rhodamine-based fluorescent dye characterized by its strong absorption, high fluorescence quantum yield, and excellent thermal and photostability. It is optimally excited within the 545-575 nm wavelength range and emits in the orange-red region of the visible spectrum. This dye is particularly suited for advanced applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays. However, due to its limited water solubility, ATTO 565 may be less ideal for aqueous-based applications. For these contexts, iFluor® 568 offers a viable alternative, providing similar spectral profiles with brighter fluorescence and improved aqueous compatibility.

The azide derivative of ATTO 565 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.